糖尿病性高血压大鼠肾损害相关基因cDNA片段的克隆与初步鉴定  

Cloning and Identification of cDNA Fragments Related to Renal Damage of Diabetic Hypertension Rats

作　　者：张芳林[1] 李果[1] 叶传忠[2] 丁伟[1] 刘优萍[1] 谢超[1] 张迪[1] 孙卫华[1] 罗敏[1] 

机构地区：[1]上海第二医科大学附属瑞金医院,上海市内分泌研究所上海200025 [2]复旦大学附属中山医院泌尿外科,上海200032

出　　处：《生物化学与生物物理进展》2002年第1期94-99,共6页Progress In Biochemistry and Biophysics

摘　　要：为筛选大鼠糖尿病性高血压病所致的肾损害相关基因 ,自发性高血压大鼠以STZ 5 0mg/kg一次性尾静脉注射 ,建立糖尿病性高血压大鼠模型 ,4周后该模型大鼠尿蛋白持续阳性 ,电镜观察到肾小球基质增生、足突融合或扁平 .应用荧光标记的差异显示反转录聚合酶链反应 (DDRT PCR)及RNA印迹技术 ,比较两种模型大鼠肾皮质的基因表达差异 ,并进行差异条带的cDNA序列生物信息学分析 .其中 4个差异条带与Genbank数据库比较 ,2个为未知基因 ,2个为已知基因 ,即成淀粉样糖蛋白 (amyloidogenicglycoprotein ,AGG)、CDK10 9,两者可能与糖尿病性高血压肾损害的发生相关 ,对它们的进一步研究将为肾损害发病机理的发现提供思路 .To investigate new genes related to renal damage of diabetic hypertension rats for exploring its mechanism, the model of diabetic hypertension rats was established by streptozotocin (STZ) injection. Urine protein of this model rats continued positive and ultrastructure of their renal cortex changed. Fluorecence-labelled differential display reverse transcription polymerase chain reaction and Northern blot were used to isolate and identify the genes that showed transcription changes in the renal cortex between the unitary hypertension and diabetic hypertension rats. Four differential fragments were obtained, two of which were novel whereas the other two showed significant similarity with rat amyloidogenic glycoprotein and CDK109 respectively according to the public database of Genbank. Via the application of bioinformatics the altered renal mRNA expression of these genes associated with diabetic hypertension suggested that they were the candidates for a role in the development of the nephropathy.

关 键 词：差异显示 糖尿病性高血压 肾损害 生物信息学 相关基因 CDNA片段 克隆 

分 类 号：R544.14[医药卫生—心血管疾病] R587.2[医药卫生—内科学]