与丙型肝炎病毒复制中间体3′末端结合的宿主蛋白的研究  

A Cellular Protein p45 Specifically Binds to The 3′-end of Hepatitis C Virus Intermediate Negative-strand RNA

作　　者：邓庆丽[1] 黄开红[1] 邵静[1] 黄志明[1] 王巍[2] 

机构地区：[1]中山医科大学孙逸仙纪念医院医学研究中心,广州510120 [2]中山医科大学肿瘤防治中心内科,广州510060

出　　处：《生物化学与生物物理进展》2002年第1期133-138,共6页Progress In Biochemistry and Biophysics

基　　金：广东省自然科学基金资助项目 (990 098;990 1 0 1 )~~

摘　　要：宿主细胞蛋白在HCV复制过程中起着重要的作用 .应用蛋白质 核酸紫外交联法检测多个细胞株 ,目的是明确是否存在可与HCV复制中间体 3′末端特异性结合的细胞蛋白质 .结果显示 ,在多个细胞株中存在一个分子质量约为 45ku的蛋白质 (命名为 p45 ) ,可与HCV复制中间体 3′末端 131～ 2 78nt结合 ,这种结合可被过量的自身未标记RNA竞争抑制 ,而非同源蛋白和非同源RNA均不能竞争抑制这种结合 .根据计算机RNA二级结构分析推测 ,p45可能特异性结合于HCV复制中间体 3′端的一茎环结构内 .这一结果提示 ,p45在HCV子代RNA复制中可能起着重要作用 .In order to study the possible mechanism of the cellular proteins involved in the process of replication of HCV RNA. UV cross-linking experiment was used to identify the cellular proteins which bind to the 3'-end of HCV intermediate negative-strand RNA. Results showed that a protein with approximate molecular mass of 45 ku (p45) cross-linked with the 3'-end 131 similar to 278 nt of HCV intermediate negative-strand RNA. The p45 presented in various cell lines of different origins. The,amounts of RNA-protein complexes increased with increasing amounts of cellular extracts. Non-homologous proteins and RNA transcripts could not compete for the binding between p45 and the 3'-end of HCV intermediate negative-strand RNA. These results suggested that the cellular protein p45 can specifically bind to the secondary structure of the 3'-end of HCV intermediate negative-strand RNA, thus p45 may play an important role in HCV RNA replication.

关 键 词：丙型肝炎病毒 复制体 蛋白质-核酸紫外交联试验 宿主蛋白 

分 类 号：R373.21[医药卫生—病原生物学]