巴西橡胶树Polyubiquitin基因5'调控序列的克隆及分析  

Cloning and Sequence Analysis of 5' Regulatory Region for Polyubiquitin Gene fromHevea brasiliensis

作　　者：彭世清[1] 陈守才[1] 

机构地区：[1]中国热带农业科学院热带作物生物技术国家重点实验室,海南海口571101

出　　处：《农业生物技术学报》2002年第1期56-59,共4页Journal of Agricultural Biotechnology

基　　金：中国热带农业科学院科技基金资助项目

摘　　要：利用GenomeWalker的方法获得了巴西橡胶树Polyubiquitin基因的5’调控序列。序列分析表明,在第一个Ubiquitin的起始密码子前含有1个内含子,内含子含有1175bp,A+T含量为71.1％;另外存在TATA box、CAAT motif、Gbox、HSE以及TCA-like等一些调控元件和1个约含230bp的A+T含量丰富（90％）的区域。The 5' end of polyubiquitin gene from Hevea brasiliensis was obtained by GenomeWalker mefhod. It contained an 1175 bp intron immediately 5' to the initiation code for the first ubiquitin-coding. Several sequences similar to eukaryotic cis regulatory element were found in the 5'-UTR 430 bp proximal 5' flanking sequence. A putative TATA box sequences found at -33bp. At position -79 bp, a TCAAT sequence could be observed, which is similar to the 'CCAAT' motif found in a multitude of higher eukaryotic promoters. The G-box element sequence, CACGTG, was found at -127 bp. Some sequences 5'-nGAAn-3' present many positions, which is similar to the consensus heat-shock element. The A+T rich region, containing 90% A+T in the 230bp sequence, is present in the 5' flanking region.

关 键 词：巴西橡胶树 POLYUBIQUITIN 5′调控序列 基因克隆 

分 类 号：S794.1[农业科学—林木遗传育种]