原始生殖细胞的人类ES细胞的分离克隆  

Isolation and Culture Human Embryonic Stem-like Cells Derived from Primordial Germ Cells

作　　者：华进联[1] 窦忠英[1] 李松[1] 李相运[1] 雷安民[1] 杨春荣[1] 高志敏[1] 张若平 

机构地区：[1]西北农林科技大学家畜生殖内分泌与胚胎工程农业部重点开放性实验室,陕西杨凌712100 [2]西安市中医医院,陕西西安710000

出　　处：《农业生物技术学报》2002年第1期64-66,T001-T006,共9页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(39970363);国家973项目(G1999054300)资助

摘　　要：本研究收集47例4～13周龄人流产胎儿分离PGCs,采用与其生殖嵴/腺或类似物胎儿成纤维细胞共培养的方式,获得了具小鼠ES细胞或EG细胞特征的人类ES细胞。该类细胞在体外可自发分化为类胚体、成纤维细胞样细胞、神经胶质细胞样细胞及心脏跳动样细胞团等。同样方法自4例大于17周龄胎儿未观察到人类ES细胞。研究表明,采用共培养法可以从4～13周龄流产儿的PGCs分离克隆到人类ES细胞,最适宜胎龄为7～10周龄。Human embryonic stem-like cells (ES-like cells) with the charactistics of mouse ES cells or embryonic germ (EG) cells derived from gonad, gonadal ridge and mesenteries, analogy or the surrounding tissues of 4~13 weeks postfertilization human embryos.were observed These cells in vitro could differentiate into fibroblast-like cells,astrocyte-like cells, heartbeat-likecell masses. No ES-like cell colonies were observed derived from 4 foetuses with the age of 4~6 months.It indicates that human ES-like cells can be isolated and cultured by coculturing with embryonic fibroblast of the same foetus from primordial germ cells of 4~13 weeks postfertilization human embryos. The ideal age of human embryos for ES cells is 7~10 weeks.

关 键 词：人 原始生殖细胞 胚胎干细胞 胚胎生殖细胞 ES细胞 分离 克隆 

分 类 号：R321－33[医药卫生—人体解剖和组织胚胎学] R321.1[医药卫生—基础医学]