人类轮状病毒基因DNA免疫的初步研究  

Primary Studies on DNA Immunization of Human Rotavirus

作　　者：彭世勇[1] 王健伟[1] 温乐英[1] 何金生[1] 洪涛[1] 

机构地区：[1]中国预防医学科学院病毒学研究所,北京100052

出　　处：《中国病毒学》2002年第1期38-41,共4页Virologica Sinica

基　　金：国家863计划生物技术领域资助课题(2001AA215011)。

摘　　要：本文研究人类轮状病毒基因ＤＮＡ免疫及应用。通过构建重组质粒ｐＣＩ／ｖｐ７，ｐＣＩ／ｖｐ４及ｐＣＩ／ｖｐ６，以肌注法导入ＢＡＬＢ／ｃ小鼠肌肉组织，其中ｐＣＩ／ｖｐ７及ｐＣＩ／ｖｐ４能　有效引起机体免疫应答，而且对轮状病毒Ｗａ株有一定中和效应。从本次试验的结果来看　，人类轮状病毒ＤＮＡ疫苗能作为预防病毒性小儿腹泻的一种手段。The probability of DNA vaccine against human rotavirus wa s studied, by constructing three recombinant plasmid clones, pCI/vp7, pCI/vp4, a nd pCI/vp6, and transferring into TA muscle(quadriceps) of BALB/C mouse with intr amuscular inoculation. The sera were collected and antibody activity was evaluat ed with ELISA and neutralizing test. pCI/vp7, pCI/vp4, and pCI/vp6 have been suc cessfully Constructed, moreover, pCI/vp7 has transfected 293 cells and the vp7 gene could express thoroughly. Intramuscular inoculation of these plasmids induced ef ficient immuniation, and the antibody induced by pCI/vp7 presents some definite protective of a strain of rotavirus Wa. As the results showed, DNA vaccine will b e a helpful means to protect children from severe acute diarrhea caused by Group A rotaviruses.

关 键 词：重组质粒 DNA免疫 人类轮状病毒 

分 类 号：R392[医药卫生—免疫学]