桃蚜体内与病毒结合的共生菌Buchnera　groEL基因的克隆和序列分析  

Cloning and Sequence Analysis of Buchnera GroEL Gene from Endosybiotic Bacterium of Myzus persicae

作　　者：崔晓峰[1] 吴云锋[1] 林林[1] 梁小波[1] 张建新[1] 周广和[2] 

机构地区：[1]西北农林科技大学植保学院,陕西杨凌712100 [2]中国农业科学院植物保护研究所,北京100094

出　　处：《中国病毒学》2002年第1期65-72,共8页Virologica Sinica

基　　金：国家自然科学基金(39970483);国家863青年基金(97-137)

摘　　要：以桃蚜（Ｍｙｚｕｓ　ｐｅｒｓｉｃａｅ）杨凌生物型为材料，利用一对特　异性引物　，用ＰＣＲ的方法从桃蚜体内扩增出了内共生菌的Ｂｕｃｈｎｅｒａ　ｇｒｏＥＬ基因，序　列　测定结果表明其长度为１　６４７ｂｐ，与ＧｅｎＢａｎｋ中的桃蚜荷兰生物型、蜿豆蚜（Ａｃｙｒｔｈｏｓｉｐｈｏｎ　　ｐｉｓｕｍ）日本生物型Ｂｕｃｈｎｅｒａ　ＧｒｏＥＬ基因长度相同，同源性分别为９９％和９１％；Ｂｕｃｈｎｅｒａ　ＧｒｏＥＬ－ＹＬ编码５４８个氨基酸，序列比较发现与Ｂｕｃｈｎｅｒａ　ＧｒｏＥＬ－ＮＴ仅有３个氨基酸的差异，即ＡＡ１１１Ｍｅｔ→Ｌｙｓ、ＡＡ２２２Ｖａｌ→Ｍｅｔ　ａｎｄ　ＡＡ３４８Ｇｌｎ→Ｈｉｓ．Buchnera groEL gene of endosymbiotic bacterium (Buc hnera sp.) from Myzus persicae Yangling boitype (P.R.China) was isloated,a n d the complete nucleotides sequence was determined.It is indicated that Buchne ra groEL gene of M.persicae includes 1647 base pairs,has 99% and 91% sequ ce similarity with Buchnera groEL gene of M.persicae The Nether land bi otype (AF003957) and Acyrthosiphon pisum Japanese biotype (X61150),respectiv ely.Buchnera GroEL-YL contains 548 amino acide,and has 3 different amino ac ids comparing with Buchnera GroEL-NT,that is AA111Met→Lys、AA222Val→Met and AA348Gln→His.

关 键 词：桃蚜 内共生菌 传毒 基因克隆 序列分析 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治]