猪瘟病毒野毒株持续感染细胞模型的建立  

Model Establishment of the Cell Persistently Infected bya Field-isolated Virulent Strain of CSFV

作　　者：应松成[1] 郑从义[1] 屈三甫[1] 张楚瑜[1] 王在时[2] 

机构地区：[1]武汉大学生命科学学院,湖北武汉430072 [2]中国兽药监察所,北京100081

出　　处：《中国病毒学》2002年第1期77-81,共5页Virologica Sinica

基　　金：国家重点基础研究发展规划项目"973"(G1999011904)

摘　　要：采用猪瘟病毒野毒珠（ＣＳＦＶ３９）感染猪肾传代细胞系ＰＫ－１５，经连续传７９代，建立了稳定的病毒持续感染细胞模型，获得ＣＳＦＶ３９－ＰＫ１５细胞株。用免疫荧光、ＲＴ－ＰＣＲ检测、透射电镜跟踪观察了ＣＳＦＶ３９－ＰＫ１５细胞株连续传代中病毒在细胞内的存在情况。结果表明第９，２９，７９代细胞仍有猪瘟病毒存在，表现出病毒持续感染的基本特征。这为深入研究猪瘟病毒持续感染机理奠定了基础。A porcine kidney cell line(PK-15) was infected by a fiel d-isolated virulent strain of classical swine fever virus (CSFV39).After the in f ected cells had subcultured 79 passages for near eight months,a steady m odel of the cell persistently infected by virus has been established,hence the C SFV39-PK15 cell line was procured.In this experiment immunofluorescence,RT-PCR assa y and electron microscopy observation were used to identify the basic viral feat ures in the CSFV39-PK15 cell. The results showed the 9th,29th,79th CSFV39-PK1 5 cell passages still harbored CSFV and they had the radical characteristics of v iral persistent infection.This endeavor should be the foundation for the furthe r research of CSFV persistent infection.

关 键 词：猪瘟病毒株 CSFV39 持续感染 细胞模型 RT-PCR 

分 类 号：S852.65[农业科学—基础兽医学]