对虾白斑病毒1197基因的表达和产物纯化的研究  

The Studies on Expression of 1197 Gene of Prawn Baculovirus and on Purifications of the Expressed Product

作　　者：应智伟[1] 周国瑛[1] 龚祖埙[1] 

机构地区：[1]中国科学院上海生物化学与细胞生物学研究所,上海200031

出　　处：《中国病毒学》2002年第1期92-95,共4页Virologica Sinica

基　　金：国家科技部海洋863项目资助(863-819-04-08)

摘　　要：In previous report we have cloned a putative early and la te transcriptional gene 1197 of prawn baculovirus.In order to detect the biologi cal function of this gene,1197 gene was inserted into expressing vec tor pGEX-3X .The fusion protein expressed with high yield was obtained,but it was found that the fusion protein locates in inclusion bodies of E.coli and it caused trou ble in its purfication.We found that the expressed product with MW.of 66kD could be purified from inclusion bodies by using a serious of treatments of inclusion bodies with urea and guanidine hydrochloride and then by sequential FPLC chroma tography.In previous report we have cloned a putative early and la te transcriptional gene 1197 of prawn baculovirus.In order to detect the biologi cal function of this gene,1197 gene was inserted into expressing vec tor pGEX-3X .The fusion protein expressed with high yield was obtained,but it was found that the fusion protein locates in inclusion bodies of E.coli and it caused trou ble in its purfication.We found that the expressed product with MW.of 66kD could be purified from inclusion bodies by using a serious of treatments of inclusion bodies with urea and guanidine hydrochloride and then by sequential FPLC chroma tography.

关 键 词：对虾白斑病毒 1997基因 表达产物 纯化 

分 类 号：S945[农业科学—水产养殖]