猪链球菌2型国内分离株毒力相关蛋白的分析  

Analysis of Virulence-Related Proteins of Streptococcus suis Type 2 from Swine Streptococcus Isolatrd in China

作　　者：欧瑜[1] 陆承平[1] 

机构地区：[1]南京农业大学农业部动物疫病诊断与免疫重点实验室,南京210095

出　　处：《微生物学报》2002年第1期105-109,共5页Acta Microbiologica Sinica

基　　金：国家 973项目子课题 (G1 9990 1 1 90 6 )

摘　　要：提纯猪链球菌 2型江苏分离株HA980 1的毒力相关蛋白溶菌酶释放蛋白 (muramini dase releasedprotein ,MRP)和胞外因子 (extracellularfacter,EF) ,制备其抗体供免疫转印之用。另提取猪源链球菌 1 7株国内分离株、1株德国分离株及 1株猪链球菌 2型人分离株的胞壁和胞外蛋白 ,经SDS PAGE ,与HA980 1株的MRP和EF的抗体作免疫转印。 1 1株MRP阳性 ,1 0株EF及EF 阳性 ,MRP及EF的分布存在 4种表型 :MPR+ EF+ (8 1 9) ,MRP+ EF (1 1 9) ,MRP+ EF- (1 1 9) ,MRP- EF- (1 0 1 9)。Virulence-related proteins, muraminidase-released protein (MRP) and extracellular factor (EF) of Streptococcus suis type 2, were extracted from Jiangsu Isolate HA9801 and were used as antigens for preparation of antibodies. Bacterium cell envelope proteins and extracellular proteins of swine Streptococcus strains including 17 Chinese isolates, 1 German strain and 1 human isolate of Streptococcus suis type 2, were analyzed by SDS-PAGE and immunoblotting using the above antibodies, 11 strains produced MRP and 10 strains possessed EF or EF *. They exist four phenotypes:MRP +EF +(8/19), MRP +EF *(1/19),MRP +EF -(1/19),MRP -EF -(10/19).

关 键 词：猪链球菌 溶菌酶释放蛋白 胞外因子 毒力相关蛋白 

分 类 号：S858.282.6[农业科学—临床兽医学]