XCAP-C-like Protein Existing in Nuclei and Chromosomes of Allium sativa  

XCAP-C类似蛋白存在于蒜根端细胞核和染色体中(英文)

作　　者：王岩[1] 邢苗[2] 阎石[3] 

机构地区：[1]东北师范大学遗传与细胞研究所,长春130024 [2]深圳大学,深圳518060 [3]中国人民解放军军需大学,长春130062

出　　处：《Acta Botanica Sinica》2002年第1期29-33,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金 ( 39870 389);国家教育委员会跨世纪人才计划基金~~

摘　　要：In studying the XCAP-C-like protein in the root meristematic cells of Allium sativa L., the nuclei were isolated from the cells and the nuclear matrices prepared. A 165 kD polypeptide, which is equivalent to XCAP-C in molecular weight, was demonstrated in the nuclei by SDS-PAGE, and was then proved to be an XCAP-C-like protein by Western blot using an anti-XCAP-C antiserum, but neither the polypeptide nor the XCAP-C-like protein was detected in die nuclear matrix. The nuclei, Chromosomes and chromosome scaffolds were observed to emanate strong, specific fluorescence after labeled with the anti-XCAP-C antiserum and an FITC-conjugated secondary antibody, indicating their containment of the XCAP-C-like protein. It was confirmed by viewing with immunoelectron microscopy that the gold particles representing the localization of the XCAP-C-like protein were found to be mainly distributed in the condensed chromatin regions of the nuclei and chromosomes.以抗XCAP_C抗体为探针 ,用SDS_PAGE、免疫印迹、免疫荧光和免疫电镜技术 ,对蒜 (AlliumsativaL .)根端细胞核、核骨架、染色体和染色体骨架进行研究。SDS_PAGE和免疫印迹结果表明 :细胞核中的 16 5kD多肽是XCAP_C类似蛋白 ,在核骨架中未检测到XCAP_C类似蛋白。免疫荧光和免疫电镜结果表明 :蒜细胞核、染色体和染色体骨架中含有XCAP_C类似蛋白 ,该蛋白位于细胞核中的染色质区域 ,但核骨架不含有XCAP_C类似蛋白。

关 键 词：Allium saliva SMC protein XCAP-C-like protein nucleus chromosome 

分 类 号：S633.4[农业科学—蔬菜学]