同腹异育银鲫对鱼类3种致病弧菌粗脂多糖的免疫应答  

Immune Response of Offsprings of Silver Crucian Carp, Caressius auratus gibelio , to Crude Lipopolysaccharide of Three Pathogenic Vibrio Bacteria

作　　者：黄辨非[1] 陈超然[1] 毛芝娟 陈昌福[1] 

机构地区：[1]华中农业大学农业部农业微生物重点开放实验室 [2]浙江省宁波市水产研究所,宁波315012

出　　处：《华中农业大学学报》2002年第1期59-63,共5页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金 (批准号 :3 9870 5 98);华中农业大学农业部农业微生物重点开放实验室开放课题;浙江省宁波市青年科技基金 (批准号 :99110 4);宁波市重点科技计划 (编号 :0 0 2 5 0 2 )资助项目

摘　　要：用从鳗弧菌 (Vibrioanguillarum )、哈维氏弧菌 (Vibrioharveyi)和副溶血弧菌 (Vibrio para haemolyticus)等 3种弧菌中提取的菌体粗脂多糖 (LPS)作为免疫原 ,分别经胸鳍基部接种同腹异育银鲫 (Caras siusauratusgibelio)后 ,通过测定受免鱼的凝集抗体效价、头肾和血液中吞噬细胞的吞噬活性和杀菌活性 ,检测 3种弧菌的粗LPS对异育银鲫免疫应答的试验结果。结果表明 ,3种弧菌的粗LPS对异育银鲫均具有较强的免疫原性 ,受免鱼的血清中出现了对 3种弧菌的福尔马林灭活菌体 (FKC)的交叉凝集抗体 ;与对照鱼相比 ,受免鱼头肾和血液中吞噬细胞的吞噬活性和杀菌活性明显上升 ,证明在 3种弧菌的粗LPS上存在共同保护性抗原 。The offsprings of silver crucian carp ( Caressius auratus gibelio ) were immunized with crude lipopolysaccharide(LPS) extracted from Vibrio harveyi , V. parahaemolyticus and V. anguillarum . The immune response of offsprings of silver crucian carp were compared with examing the agglutinating antibody titers, cross agglutinating antibody titers of serum, the phagocytic activity and bactericidal activity of phagocytes in the blood and head kidney. The results showed that the agglutinating antibody titers and cross agglutinating antibody titers of immunized offsprings of silver crucian carp were higher than the control group, and the phagocytic activity and bactericidal activity of phagocytes in the blood and head kidney of offsprings of silver crucian carp was significantly boosted and had a high level of immune response. The crude LPS of three pathogenic bacteria were thought to have a common protective antigens to cause a cross immune response to silver crucian carp.

关 键 词：鳗弧菌 哈维氏弧菌 副溶血弧菌 粗脂多糖 同腹异育银鲫 免疫原性 疫苗 

分 类 号：S941.42[农业科学—水产养殖] S942.5[农业科学—水产科学]