致敏小鼠抗原激发后肺局部免疫反应的研究  

The observation of local immune response in the lungs from antigen-sensitized and challenged mice

作　　者：沈华浩[1] 刘进[1] 刘富光[1] 王选锭[1] 林小莉[1] 徐志豪[1] 黄刚[1] 原双晋[1] 

机构地区：[1]浙江大学医学院附属第二医院呼吸科,杭州310009

出　　处：《中华结核和呼吸杂志》2002年第3期156-158,共3页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的　探讨抗原致敏和激发后肺局部免疫反应的变化。方法　以抗原钥孔嘁血兰素(KLH)经气管内滴注致敏小鼠 ,2～ 4周后进行激发 ,期间分别收集支气管肺泡灌洗液 (BALF)和外周血 ,对肺、肺相关淋巴结 (LALN)和脾组织细胞悬液进行培养并收集上清液 ,以酶联免疫吸附试验(ELISA)测定总IgA、抗KLHIgA及白蛋白。结果　KLH致敏和激发均引起BALF中抗KLHIgA反应。抗原致敏和激发后BALF中抗KLHIgA/白蛋白比率明显高于血清 ;抗原致敏激发后肺和LALN细胞悬液在体外均可释放抗KLHIgA ,而脾细胞悬液仅释放低水平抗KLHIgA。结论　气管内滴注抗原致敏小鼠可诱导肺局部抗原特异IgA反应 ,抗原激发后反应加强。肺局部积聚的抗KLHIgA并非由血中渗漏而来 ,而是局部产生的结果。肺和LALN中的淋巴细胞是抗原特异IgA的主要来源。Objective To evaluate the local immune response after allergic sensitization and challenge in the lungs of mice.Methods C57BL/6 mice were sensitized with keyhole limpet hemocyanin (KLH) by intratracheal instillation and challenged 2～4 weeks later. Bronchoalveolar lavage fluid (BALF) and plasma were collected and cells from the lung associated lymph node (LALN), the lungs and the spleen were cultured and collected.Anti KLH IgA, total IgA and albumin levels were measured by ELISA.Results Intratracheal instillation of KLH induced local BAL antigen specific IgA response, and further challenge expanded this response. The ratio of anti KLH IgA/albumin in BALF was significantly higher than that in serum after both sensitization and challenge by KLH. In vitro, cells from the LALN and the lungs released anti KLH IgA after sensitization and challenge by KLH, but spleen cells released lower levels of anti KLH IgA compared to the LALN and the lungs. Conclusions Allergic sensitization by intratracheal instillation of KLH into the mouse lungs induced a local pulmonary response of specific IgA, and it was amplified by challenge with KLH. The accumulation of anti KLH IgA in the respiratory lumen was the result of local production but not the result of simple transudation or leakage from the serum. The LALN and the lung lymphocytes were major sources of anti specific IgA.

关 键 词：肺 抗原 免疫反应 动物实验 LALN 淋巴细胞 IGA 呼吸道感染 

分 类 号：R563.9[医药卫生—呼吸系统]