毛叶枣APETALA1同源基因的克隆  

Molecular Cloning of APETALA1 Gene from Zizyphus mauritianan Lam.

作　　者：胡桂兵[1] 徐昌杰[1] 陈大成[2] 郑启发[2] 安新民[1] 张上隆[1] 

机构地区：[1]浙江大学园艺系,浙江杭州310029 [2]华南农业大学园艺系,广东广州510642

出　　处：《华南农业大学学报》2001年第3期43-46,共4页Journal of South China Agricultural University

基　　金：华南农业大学陈励文基金资助项目 (5 30 0 -k990 47)

摘　　要：分析在植物花分生组织及花器官形成过程中起重要作用的APETALA1(AP1)基因的保守区序列 ,设计 1对长度为 2 3bp的PCR引物 ,以毛叶枣基因组DNA为模板 ,采用PCR方法扩增出长为 648bp的DNA片段 ,克隆入pUCm T载体 ,测序和序列分析结果表明获得了毛叶枣AP1同源基因中部的 1个片段 ,该片段有 2个内含子 ,长度分别为15 2bp和 386bp ,编码区共编码 36个氨基酸 ,其序列已在GenBank中登记 (登记号为AF35 65 41) .在GenBank中进行同源性检索 ,结果表明其氨基酸序列与其他植物AP1同源基因的氨基酸序列同源性高达 66 %～ 88% 。A pair of 23 bp primers, designed according to a conserved sequence of the APETALA1(AP1) gene which has an important function in floral development, were used to amplify a 648 bp fragment by polymerase chain reaction(PCR). The fragment was cloned into pUCm T vector, and then sequenced. A fragment in the middle of AP1 homologous gene named ZM AP1 gene was obtained. The result of sequence analysis indicated that there were two introns of 152 bp and 386 bp in the fragment, and the exons encoded 36 amino acids. The ZM AP1 gene has been registered in GenBank with the accession number AF356541. After the deduced amino acid sequence of the fragment was submitted to GenBank and blast with AP1 homologous gene of other plants, it was found that the homology reached 66%-88%. This result suggested that ZM AP1 gene might have the same function as other AP1 homologous gene.

关 键 词：毛叶枣 AP1基因 分子克隆 APETALAI 同源基因 枣 

分 类 号：S665.1[农业科学—果树学]