伪狂犬病病毒Fa株gp63(gI)基因核苷酸序列测定及分析  

Sequence and Analysis of gp63 Gene of Pseudorabies Virus Fa-Strain

作　　者：周煜[1] 郭万柱[1] 汪铭书[1] 颜其贵[1] 

机构地区：[1]四川农业大学动物生物技术中心,四川雅安625014

出　　处：《中国兽医学报》2001年第4期317-320,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目 (3 95 70 5 44) ;国家"九五"重点攻关项目 (96 -C0 1-0 4-0 3 )

摘　　要：对我国最早分离的伪狂犬病病毒 ( pseudorabies virus,PRV) Fa株糖蛋白 gp6 3( g I)基因核苷酸序列及推导的氨基酸序列作了测定与分析。完整的 gp6 3( g I)结构基因从起始密码子 ATG到终止密码子 TGA共有1 0 50个核苷酸残基 ,编码由 34 9个氨基酸残基构成的多肽链。 4种核苷酸残基的构成比分别为 :G35.9% ,A1 1 .33% ,T1 1 .81 % ,C4 0 .95% ,G+C含量达 76 .85%。PRV Fa株 gp6 3基因核苷酸序列与 Nice株的相比 ,两者同源性达 98.1 3% ,仅存在 3个核苷酸残基的缺失变异 ;氨基酸推导序列分析表明 ,糖蛋白 gp6 3具有典型膜蛋白特征 ,与 Nice株相比 ,同源性为 97.4 2 %。 gp6 3基因起始密码子由 3个连续的 ATG组成 。The gp63 gene of the Fa strain of pseudorabies virus was sequenced by the chain termination method.The results showed that the entire sequence of gp63 gene(ORF) has 1 050 nucleotides coding a polypeptide of 349 amino acids which is characteristic of membrane protein.The overall base composition of gp63 gene of Fa strain is 35 9% guanine,11 33% adenine,11 81% thymine,40 95% cytosine,and the (G+C)% is up to 76 85%.The nucleotide and amino acid sequences of Fa strain gp63 gene were compared with the corresponding sequences of Nice strain,the overall nucleotide and amino acid homology of Fa with Nice being 98 13% and 97 42% respectively.The deletion of 3 bp in the ORF of gp63 gene locating at 514 516 nt occurred.There is no sequence in the gp63 gene which acts as a promoter.

关 键 词：伪狂犬病病毒 Fa株 gp63基因 核苷酸序列 重组质粒 

分 类 号：S855.3[农业科学—临床兽医学]