新城疫病毒地方分离株HN基因的序列分析  

Nucleotide Sequence Analysis for HN Gene of NDV Isolate

作　　者：李燕[1] 陈溥言[1] 王恩秀[1] 

机构地区：[1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室,江苏南京210095

出　　处：《中国兽医学报》2001年第4期324-326,共3页Chinese Journal of Veterinary Science

摘　　要：使用 RT-PCR的方法扩增得到有代表性的地方分离株新城疫病毒 ( NDV)的保护性抗原 HN基因。经序列测定及分析发现 ,该毒株 HN基因与日本 1 985年分离的 CHI/85株亲缘关系最近 ,氨基酸同源性达97.0 % ,与中国标准强毒株 F48E8氨基酸同源性为 89.8%。其抗原位点 发生突变 ,在 34 7位由 Glu变异为Gly。系统进化树归于The HN gene of the NL/96 strain of NDV was amplified by the reverse transcription polymerase chain reaction(RT PCR) technique using a pair of specific primer.Then the HN gene was sequenced.The enzyme sites were analyzed,and the nucleotide and amino acid sequences of HN genes of NL/96 strain were compared with those of other 13 strains.The result shows that NL/96 strain has the highest amino acid homology with CHI/85 strain,reaching 97.0%.But for NL/96 strain,an amino acid in 347 site I was changed from Glu to Gly.In evolutionary tree based on Ks value,NL/96 strain belongs in group C.

关 键 词：新城疫病毒 地方分离株 HN基因 序列分析 

分 类 号：S856.3[农业科学—临床兽医学]