腺胃病变型鸡传染性支气管炎病毒强毒株的培育  

Development of a Virulent Avian Infectious Bronchitis Virus(IBV) Causing Proventriculus Lesions

作　　者：荣骏弓[1] 吴东来[1] 谷守林[1] 王丽滨[1] 尹训南[1] 胡守萍[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001

出　　处：《中国兽医学报》2001年第4期327-330,共4页Chinese Journal of Veterinary Science

基　　金：哈尔滨市科技攻关计划项目 (9912 2 110 2 8)

摘　　要：用腺胃病变型鸡传染性支气管炎病毒分离株 IBV-D971株接种 1日龄 SPF鸡 ,连续传 1 0代后 ,培育出了腺胃病变型鸡传染性支气管炎强毒株 IBV-D971 J株。 IBV-D971 J1 0 对 SPF鸡胚的致病力为 1 0 - 6 .45ELD5 0 /0 .2 m L,对 1日龄 SPF鸡的致病力为 1 0 - 1 .5 LD5 0 /1 m L。从死亡鸡的心、肝、脾、肺、肾、腺胃、肌胃、法氏囊等均能分离到 IBV,IBV-D971 J1 0 不含鸡新城疫、禽流感、鸡马立克氏病、鸡传染性法氏囊炎、网状内皮组织增殖病等外源病毒。对 SPF鸡可引起典型的腺胃炎、肌胃炎。After 10 passages through 1 day old SPF chicks with IBV D971,a virulent strain IBV D971J was developed.The virulence of the passage 10 IBV D971J(IBV D971J 10 ) was up to 10 -6.45 ELD 50 /0.2 mL or 10 -1.5 LD 50 /mL in 1 day old SPF chicks.IBV,but not NDV,AIV,MDV,IBDV or REV,was re isolated from heart,liver,spleen,lung,kidney,proventriculus,muscle ventriculus and Fabricus bursa of the inoculated chicks.Typical pathological changes,proventriculustis,muscle ventriculus inflammation and interstitial nephritis were observed in the IBV D971J inoculated chicks.

关 键 词：鸡传染性支气管炎病毒 腺胃病变型 强毒株 鸡病 

分 类 号：S858.316.3[农业科学—临床兽医学]