致病性嗜水气单胞菌气溶素基因PCR检测方法的建立  

Detection of the Aerolysin Gene in Aeromonas hydrophila by the Polymerase Chain Reaction

作　　者：卢强[1] 任瑞文[1] 王文东[1] 刘明远[1] 吴秀萍[1] 李学勤[1] 

机构地区：[1]解放军军需大学动物科技系,吉林长春130062

出　　处：《中国兽医学报》2001年第4期347-349,共3页Chinese Journal of Veterinary Science

基　　金：总后卫生部青年基金资助项目 (96 Q 0 92 ) ;国家自然科学基金资助项目 (3 0 0 70 5 87)

摘　　要：根据我国致病性嗜水气单胞菌分离株 AH-78-2气溶素 ( Aer)基因保守区的测序结果 ,设计 2对引物 ,建立了检测嗜水气单胞菌的 PCR方法。通过对 2 0株嗜水气单胞菌、1 2株相关菌株及 1 57份送检病料的检测 ,并与 SPA-Co A检测结果对照表明 ,PCR方法具有较高的敏感性与特异性 ,可检测最低 1 0 0 cfu的细菌。该方法的建立为致病性嗜水气单胞菌的检测提供了一种简便、快速的新途径 。Synthetic oligonucleotide primers were used in a polymerase chain reaction (PCR) technique to detect the gene for aerolysin in strains of Aeromonas hydrophila and to screen for identical genes in other related bacteria strains. Primers targeted a 421 bp fragment of the aer gene coding the beta hemolysin and detected template DNA in the PCR using DNA or cells from 20 hemolytic strains of A. hydrophila. PCR amplification of DNA and /or cells from 12 other bacteria strains was consistently negative. The detection limit for the aerolysin gene by PCR amplifiction was 100 cfu of bacteria cells. The PCR clearly identified aerolysin producing strains of A. hydrophila and may be used as a species specific virulence test. The results for detection of clinical isolates of A. hydrophila suggest that the aer gene commonly present in aeromonas.

关 键 词：嗜水气单胸菌 Aer基因 检测 PCR 汽溶素基因 

分 类 号：S852.61[农业科学—基础兽医学]