约氏疟原虫Pys25、Pys21抗原的表达及其传播阻断免疫的研究  

Studies on the expression of Pys25 and Pys21 of Plasmodium yoelii and transmission blocking immunity

作　　者：刘英杰[1] 曹雅明[2] 闫建忠[2] 

机构地区：[1]中国医科大学免疫病原生物学教研室,沈阳110001 [2]中国医科大学免疫学教研室,沈阳110001

出　　处：《中国免疫学杂志》2002年第2期85-87,共3页Chinese Journal of Immunology

摘　　要：目的 :观察约氏疟原虫有性发育阶段虫体抗原Pys2 5、Pys2 1的表达及其单克隆抗体的传播阻断效应。方法 :分别用抗Pys2 5和抗Pys2 1单抗 (McAb4和McAb10 )被动免疫感染约氏疟原虫的小鼠 ,通过直接蚊喂养试验 ,观察抗Pys2 5McAb4和抗Pys2 1McAb10对疟原虫在蚊体内发育过程的影响 ;应用间接免疫荧光和Westernblotting试验 ,检测疟原虫有性发育阶段虫体抗原Pys2 5和Pys2 1表达的动态变化。结果 :与抗Pys2 1McAb10比较 ,抗Pys2 5McAb4具有更完全的传播阻断效应。Pys2 5和Pys2 1抗原蛋白存在于从配子体到动合子的整个发育过程中 ,但Pys2 5在合子表面的表达显著早于Pys2 1。结论 :约氏疟原虫Pys2 5和Pys2 1均是传播阻断免疫的靶抗原 ;抗Pys2 5McAb4具有更强的传播阻断活性与Pys2 5在合子表面的早期表达有关。Objective:To investigate the expression of antigens in Plasmodium yoelii sexual stage and transmission blocking effects of monoclonal antibodies(McAbs).Methods:Observing the effects of anti Pys25 McAb4 and anti Pys21 McAb10 on developmental course of parasites in mosquitoes by direct mosquito feeds on passively immunized P.yoelii infected mice and the expression of Pys21 and Pys25 from gametocytes to ookinetes in indicated culture times by IFA and Western blotting.Results:The transmission blocking activity of the anti Pys25 McAb4 was complete and more potent than that of the anti Pys21 McAb10.Both Pys25 and Pys21 were presented in whole developmental course from gametocytes to ookinetes.Furthermore,the expression of Pys25 appeared to be earlier than that of Pys21 on zygote surfact.Conclusion:Pys25 and Pys21 are target antigens of transmission blocking immunity and that anti Pys25 McAb4 has more significantly transmission blocking activity is related with the early stage expression of Pys25 on zygote surface.

关 键 词：约氏疟原虫 有性阶段抗原 单克隆抗体 传播阻断 Pys25 Pys21 疟疾疫苗 

分 类 号：R382.31[医药卫生—医学寄生虫学]