三七总甙对人肾成纤维细胞的影响  

Effect of Panax Notoginseng Saponins on Human Kidney Fibroblast

作　　者：韦颖[1] 樊均明[2] 潘丽萍[1] 

机构地区：[1]云南省红十字会医院肾内科,昆明650021 [2]四川大学华西医院肾内科

出　　处：《中国中西医结合杂志》2002年第1期47-49,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：云南省卫生厅科研基金资助项目 (No .99M1 2 5)

摘　　要：目的 :研究三七总甙 (PNS)对人肾成纤维细胞 (KFB)增殖、分泌I型胶原、表达整合素 β1的影响。方法 :体外培养KFB ,分别采用四甲基偶氮唑蓝 (MTT)法、ELISA法、流式细胞仪检测PNS对KFB增殖、分泌Ⅰ型胶原及表达整合素β1的影响。结果 :PNS在最佳浓度范围和作用时间内可明显抑制KFB细胞增殖及分泌Ⅰ型胶原 (与空白对照组比较 ,P <0 0 5 ) ,同时显著降低了KFB上整合素β1的表达 (与空白对照组比较 ,P <0 0 5 )。结论 :PNS可成为防治肾间质纤维化的有效药物。To study the effect of Panax notoginseng saponi ns (PNS) on cell proliferation, type Ⅰ collagen secretion and integrin β 1 ex pression in human kidney fibroblast (KFB). Methods: KFB were cultured and stimulated by PNS in vitro. The cell proliferation, type Ⅰ collagen secretion and integrin β 1 expression in KFB were measured by methyl thiazolyl tetrazolium (MTT), enzyme linked immunoa bsorbent assay (ELISA) and flowcytometry respectively. Results: Within its optimal concentration range and effective time range, PNS could obviously inhibit the proliferation, type Ⅰ collagen secr etion and integrin β 1 expression (all P<0 05) in human KFB. Conclusion: PNS would possibly be an effective drug for renal interstitial fibrosis prevention and treatment.

关 键 词：三七总甙 肾间质纤维化 整合素Β1 肾成纤维细胞 肾小管 动物实验 

分 类 号：R285.5[医药卫生—中药学]