补肾益智方对由A_β片段神经毒性诱导NG108-15细胞老年性痴呆模型神经递质释放影响的研究  

Effect of Bushen Yizhi Recipe on Neurotransmitter Release in A_β Segmen t Neurotoxin Induced NG108-15 Cellular Model of Alzheimer Disease

作　　者：钟振国[1] 刘茂才[2] 赖世隆[2] 高洁[2] 程淑意[2] 

机构地区：[1]广西中医学院第二附属医院神经科学研究所 [2]广州中医药大学老年脑病研究所

出　　处：《中国中西医结合杂志》2002年第1期50-53,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家"九五"攻关课题部分内容 (No .96 - 90 6 - 0 9- 0 2 )

摘　　要：目的 :观察补肾益智方对由Aβ片段神经毒性诱导的NG10 8- 15细胞老年性痴呆 (AD)模型神经递质释放的影响。方法 :利用免疫印迹检查、免疫放射活性测定及电生理学测定方法 ,观察补肾益智方含药血清处理Aβ片段神经毒性诱导的NG10 8- 15细胞后的胆碱乙酰基转移酶 (ChAT)活性、突触素蛋白(synapsin)水平及功能性突触形成率。 结果 :补肾益智方含药血清组ChAT活性和突触素蛋白水平高于正常对照血清组 ;含药血清能调节递质释放能力和提高功能性突触形成。结论 :补肾益智方具有减轻细胞对Aβ的神经毒性反应 ,表明该方能拮抗AD的病理发展 。To observe the effect of Bushen Yizhi Recipe (BS YZR) on neurotransmitter release in Aβ segment neurotoxin induced NG108-15 ce llular model of Alzheimer disease (AD). Methods: The levels of choline acetyltransferase (ChAT) activi ty, synapsin and functional synapse formation rate in the cellular model treated with BSYZR containing serum were determined by Western blot analysis, immunorad iometric assay and electrophysiologic technique. Results: BSYZR containing serum treatment could cause increase of ChAT activity and synapsin level in model cells, as compared with those in n ormal control model cells treated with non drug containing serum, it also could regulate the release capacity of transmitter and raise the functional synapse f ormation. Conclusion: BSYZR could reduce the reaction of cell to Aβ neu rotoxin, indicating that it could be antagonistic to the pathological developmen t of AD by means of raising the neurotransmitter release capacity.

关 键 词：补肾益智方 AΒ NG108-15细胞 神经递质 老年性痴呆 动物实验 中医药疗法 

分 类 号：R277.7[医药卫生—中医学]