银杏内酯B对牛主动脉平滑肌细胞增殖的影响  

INHIBITORY EFFECTS OF GINKGOLIDE B ON PROLIFERATION OF BOVINE AORTIC SMOOTH MUSCLE CELLS

作　　者：魏恩会[1] 饶曼人[1] 季宁东 陈秀英[2] 陈琪[2] 

机构地区：[1]南京医科大学心血管药理研究室,江苏南京210029 [2]南京医科大学动脉粥样硬化研究中心,江苏南京210029

出　　处：《药学学报》2002年第2期90-93,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目 (3 9870 3 63 )

摘　　要：目的　探讨银杏内酯B(GB)对牛主动脉平滑肌细胞 (SMC)增殖的影响及可能机制。方法　细胞的增殖由3H 胸腺嘧啶核苷掺入试验确定 ,应用流式细胞术测定细胞周期 ,用MTT法和乳酸脱氢酶活性检测观察细胞代谢的变化。结果　无论是否用血管紧张素II诱导 ,GB与银杏内酯A和B的混合物均呈浓度依赖性地抑制SMC的增殖 ,其机制与阻止细胞进入有丝分裂期有关。结论　GB可抑制动脉壁SMC的增殖 。AIM To investigate the effects of ginkgolide B (GB) on proliferation of bovine aortic smooth muscle cells (SMC) and its related mechanisms. METHODS After pretreating with GB or the mixture of ginkgolide A and B ( GA∶GB ) at 37℃ for 0 5 h, the VSMC were treated with or without angiotensin II (Ang II) for 24 h. The proliferation of SMC was evaluated by 3H thymidine incorporation and the cell cycle phase was measured by flow cytometry. Cytotoxicity was reflected by MTT and lactate dehydrogenase (LDH) activity of the supernatant. RESULTS Whether or not treated with Ang II, GB and GA∶GB were shown to suppress SMC proliferation in concentration dependent fashion at concentrations ranging from 10 -9 mol·L -1 to 10 -5 mol·L -1 . The inhibitory effects appeared to be related to a G 1→S block in cell cycle traverse. CONCLUSION The suppression of SMC proliferation by GB might not only be contributed by blockage of the PAF receptor activity.

关 键 词：银杏内酯B 主动脉平滑肌细胞 细胞增殖 动物实验 

分 类 号：R282.710.5[医药卫生—中药学] R965[医药卫生—中医学]