天麻和阿胶联合对染铅大鼠海马一氧化氮含量及学习记忆的影响  

Joint effect of Gastrodia elata blume and Donkey-hide gelatin on the content of nitric oxide in hippocampus and the ability of learning and memory during lead administration in rats

作　　者：李茂进[1] 胡俊峰[1] 张春玲[1] 于素芳[1] 韩惠芬[1] 

机构地区：[1]山东大学公共卫生学院,山东济南250012

出　　处：《中国职业医学》2002年第1期10-12,共3页China Occupational Medicine

基　　金：山东省科委博士基金 (编号 :41 3774)

摘　　要：目的　观察和探讨天麻和阿胶联合对染铅大鼠学习记忆损害的拮抗作用。方法　Wistar雄性大鼠经口染铅并分别单独及联合给予天麻 (每天 4 0g/kg)和阿胶 (每天 1 0g/kg)灌胃 ;每月用游泳试验测试学习记忆一次 ,3个月后处死大鼠 ,测定海马组织一氧化氮浓度。结果　在游泳试验中 ,高铅对照组和低铅对照组大鼠 5min内到达平台次数 ,与空白组比较差异有非常显著性 (P <0 .0 1) ;天麻和阿胶单用即可明显增加染铅大鼠抵达平台的次数 (P <0 .0 5 ,第2、3月P <0 .0 1) ,两者联用效果更为显著 (P <0 .0 1)。海马组织一氧化氮测定 ,高铅对照组和低铅对照组海马一氧化氮 ,与空白组比较差异均有非常显著性 (P <0 .0 1) ;天麻单用即可明显提高染铅大鼠海马一氧化氮 (P <0 .0 5 ,低铅时P<0 .0 1) ;阿胶单用亦可明显提高染铅大鼠海马一氧化氮 (P <0 .0 5 ) ;天麻和阿胶联用效果更为显著 (P <0 .0 1)。病理检查 :高铅对照组和低铅对照组大鼠海马萎缩 ,细胞变性、坏死 ,轴突断裂、溶解、消失等 ,而高铅天麻阿胶组和低铅天麻阿胶组细胞变化不明显。结论　天麻和阿胶单用即可显著减轻铅对学习记忆的损害作用 ,两者联用效果更加显著。Objective To observe and explore the antagonistic effect of the combination of Gastrodia elata blume (GTEB) and Donkey hide gelatin (DHG) combined against the impairment of learning and memory in rats induced by lead. Methods GTEB (4.0 g/kg a day) and DHG (1.0 g/kg a day) were either given singlly or jointly to the male Wistar rats with each day lead administration totally for 3 months. The ability of learning and memory of each group was measured by swimming test every month; eventually, the rats were decapitated and nitric oxide (NO) in hippocampus tissue was measured immediately. Results In swimming test, the number of anchor seeking group lead decreased more significantly than that of the blank control ( P<0.01 ). Single dosing with GTEB or DHG appeared to be significantly increased the number of anchor seeking ( P <0.05, the second and the third month P <0.01). The increase of number of anchor seeking induced by GTEB and DHG combined in lead to treat rats was much more significant than that induced by GTEB and DHG monopolized ( P <0.001). The content of NO in hippocampus in the rats of lead controls was significantly decreased compared with that of the blank control ( P <0.01). The NO in hippocampus of lead exposed rats was significantly increased by GTEB monopolized ( P <0 05,the low level P <0.01). DHG single appeared to be also significantly increased ( P <0.05). The increase of NO of lead exposed rats induced by GTEB and DHG combined was much more significant than that induced by GTEB and DHG monopolized ( P <0.01). Pathological examination showed that appearance of oedema, denaturation, concentration and atrophy of cells with their neurofibers dissolving and necrosis in hippocampus of lead control rats but no evidence was shown in rats jointly given GTEB and DHG. Conclusion Single use of GTEB or DHG might significantly decrease the impairment of learning and memory induced by lead, but their joint effects appeared to be much more significant.

关 键 词：铅 天麻 阿胶 一氧化氮 学习记忆 海马 动物实验 职业中毒 

分 类 号：R135.11[医药卫生—劳动卫生] R282.71[医药卫生—公共卫生与预防医学]