卵清白蛋白特异性T细胞免疫诱导BALB/c小鼠的调节性免疫应答  

T cell vaccination induces regulatory T cell response in BALB/c mice

作　　者：张霄瑜[1] 韩淑红[1] 刘相国[1] 戴振鹏[1] 朱晓辉[1] 吕平[1] 王玉俊 高晓明[1] 

机构地区：[1]北京大学医学部免疫学系,100083

出　　处：《中华微生物学和免疫学杂志》2002年第2期127-130,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的　研究T细胞免疫后正常小鼠的调节性免疫应答。方法　应用体外扩增的卵清白蛋白 (OVA)特异的T细胞克隆免疫BALB c小鼠 ,3 H TdR掺入法分析细胞增殖 ,3 H TdR标记靶细胞检测杀伤T细胞的杀伤效应 ,间接免疫荧光法分析血清中抗T细胞抗体水平。结果　T细胞免疫后能诱导BALB c小鼠产生调节性T细胞的增殖反应、对靶细胞的杀伤效应以及针对于活化的T细胞的体液免疫应答 ,并进一步降低机体对OVA抗原的应答。Objective To investigate regulatory T cell response induced by T cell vaccination. Methods BALB/c mice were vaccinated with activated OVA-specific T cell clones expanded in vitro. Cell proliferation was measured by 3H-TdR incorporation. CTL response was assayed by JAM test. Indirect immuno-fluorescent assay was used to examine the anti-T cell antibody level. Results T cell vaccination induced regulatory T cell response including T cell proliferation, CTL activity and antibody response targeting activated T cells. Conclusion Regulatory T cell response can be induced by T cell vaccination in normal mice.

关 键 词：调节性T细胞 T细胞克隆 T细胞免疫 卵清白蛋白 细胞免疫学 

分 类 号：R392[医药卫生—免疫学]