再生障碍性贫血患者淋巴细胞表型变化  

Abnormal expressed co-stimulating factors and their relationship to activation of T lymphocytes in patients with aplastic anemia

作　　者：傅晋翔[1] 於葛华[1] 张学光[2] 

机构地区：[1]苏州大学第二附属医院 [2]苏州大学生物技术研究所

出　　处：《中华微生物学和免疫学杂志》2002年第2期152-155,共4页Chinese Journal of Microbiology and Immunology

基　　金：国防研究项目 (Y5 5 732 6 2 );苏州大学医学发展基金资助项目 (Q312 30 5 6 )

摘　　要：目的　研究再生障碍性贫血 (AA)患者骨髓 (BM)及外周血 (PB)淋巴细胞及其活化相关分子的表达及临床意义。方法　采用单色和双色免疫荧光标记法、流式细胞仪分析AA患者的BM和PB中淋巴细胞膜分子的表达。结果　AA患者BM和BP中CD8+细胞增加 ,CD4 CD8比例下降 ,BM中CD2 5 +细胞和HLA DR+细胞增多 ,急性AA增加尤为显著 (P <0 .0 1) ,BM中CD16 +或CD5 6 +细胞也明显增多 (P <0 .0 5 ) ;双标记分析提示T细胞主要为CD8+细胞 ;急性AA患者CD8+ CD2 5 +细胞显著增多 (P <0 .0 1) ,AA患者BM中淋巴细胞活化相关分子表达增多 ,尤其是 4 1BB+、CD95L+和CD40L+细胞显著增多 (P <0 .0 1)。结论　AA患者BM中淋巴细胞活化相关膜分子增多 。Objective To investigate the phenotypes and expression of activated T cell membrane molecules on lymphocytes in the peripheral blood(PB) and bone marrow(BM) of the patients with aplastic anemia. Mothed Single and double colors immunoflourescence were used to analyze the mononuclear cells from PB and BM of the AA patients by flow cytometry. Results The expression of CD8 increased and ratio of CD4 + to CD8 + cells decreased significantly. The expression of CD25 and HLA-DR increased in the BM, which was more significant in patients with serious AA (P<0.01) and the CD16 or CD56 positive cells also increased in BM. Two-colors analysis showed that the most activated T cells were CD8 + cells. The CD8 +-CD25 + lymphocytes increased significantly in the BM (P<0.01). Co-stimulatory molecules which essential to activation of lymphocytes such as 4-1BB, CD95L and CD40L increased significantly in the BM of AA patients. Conclusion Increase of co-stimulating molecules which were necessary for lymphocytes activation in the BM of AA patients is one of main reason to explain abnormal T cells activation and failure of hematopoiesis.

关 键 词：再生障碍性贫血 淋巴细胞异常活化 细胞表型变化 

分 类 号：R556[医药卫生—血液循环系统疾病]