白细胞介素4在输注供体肝脏非实质细胞诱导免疫耐受中的作用  

The role of IL-4 in the induction of tolerance by pretransplant infusion of donor liver nonparenchymal cell

作　　者：丁小明[1] 薛武军[1] 纪宗正[1] 田普训[1] 潘晓鸣[1] 

机构地区：[1]西安交通大学第一医院肾移植科,710061

出　　处：《中华器官移植杂志》2002年第2期90-92,共3页Chinese Journal of Organ Transplantation

摘　　要：目的　探讨皮肤移植受体在输注供体肝脏非实质细胞 (NPC)诱导免疫耐受过程中 ,白细胞介素 4(IL 4)所起的作用。方法　将 2× 1 0 7个C3H/He (C3H)小鼠的肝脏非实质细胞通过尾静脉输入C57BL/ 6(B6)小鼠的体内 ,48h后B6小鼠腹腔注射环磷酰胺 2 0 0mg/kg ,1 8d后接受C3H小鼠皮片的移植 ,分别于NPC细胞输注前及输注后 7、1 8、30、60d采血监测IL 4水平的动态变化。结果　 1 5只接受皮片移植的小鼠 ,其皮片存活时间显著延长 ,皮片平均存活时间 (70± 1 7.2 )d ,正常对照组其移植皮肤的平均存活时间仅 (1 0± 0 .4)d ,用Elisa法检测NPC输注后不同时期IL 4水平的动态变化发现 ,B6小鼠体内血清IL 4水平逐渐升高 ,在长期耐受的小鼠中 ,其水平增加尤其显著 ,甚至成倍的提高。结论　ILObjective To investigate the role of IL 4 in the induction of tolerance by pretransplant infusion of donor liver nonparenchymal cells (NPC). Methods C57BL/6(B6) mice received intravenous injection through tail vein of 2×10 7 liver NPC from C3H mice. After 48?h, cyclophosphamide in PBS was injected intraperitoneally into B6 mice in a dose of 200?mg/kg. Eighteen days after liver NPC intravenous injection, B6 mice undertook C3H mice skin graft transplant. The serum level of IL 4 in B6 mice was detected by ELISA methods on the different time points.Results The survival time of skin graft in 15 mice prolonged obviously with the mean survival time being (70± 17.2 ) days, the mean survival time of normal control was only (10± 0.4 ) days. The ELISA results showed that after NPC infusion, the level of IL 4 was also increased, especially in the longer tolerant mice group.Conclusion The high level of IL 4 plays a critical role in inducing and maintaining immune tolerance.

关 键 词：白细胞介素4 静脉内输注 免疫耐受 皮肤移植 供体肝脏非实质细胞 

分 类 号：R617[医药卫生—外科学]