大鼠实验性脾虚证胃粘膜酶组织化学的研究  

Enzyme Histochemical Study on Gastric Mucosa of “Experimental Spleen Deficiency Syndrome” in Rats

作　　者：王秀琴[1] 杨进[1] 刘绍杰[1] 韩秀兰[1] 刘英琴[1] 朱文胜 

机构地区：[1]首都医学院组织胚胎学教研室

出　　处：《首都医学院学报》1991年第4期260-266,共7页

摘　　要：对实验性脾虚证等4组大鼠的胃粘膜进行了组织化学和一般的组织学研究.结果表明,4组的胃粘膜均未见异常的组织学变化.实验性脾虚组的 SDH、NSE、CA 酶的活性低于对照组;LDH 酶的活性高于对照组;ATPase 和 ALP 酶的活性无明显的变化.与自然恢复组相比,治疗组以上几种酶的活性基本接近于对照组的水平.显微分光光度计的测定结果与光镜半定量结果一致.结果提示,实验性脾虚组胃粘膜酶的代谢出现异常,是导致消化功能障碍的原因之一.经四君子汤治疗后,脾虚组的几种酶活性可基本恢复正常.40 adult male rats were used and divided into 4 group,i.e.normal control group, “spleen deficiency gruop”caused by rhubarb,spontaneons recovery group and therapeutic group with Chinese herbs(Si Jun Zi decoction).The stomach of the four groups were re- moved and processed for demonstration of the reactivities of succinate dehydrogenase (SDH),Lactic dehydrogenase(LDH),magnesiu m-activa ted adenosine triphoaphatase(Mg^(2+)- ATPase),alkaline phosphatase(ALP),non-specific esterases(NSE)and carbonic anhydrase (CA).The reactivities of above mentioned enzymes in gastric mucosa were observed with light microscopy and quantitative analyses were performed.The present study revealed that the reactivities of SDH,CA and NSE in parietal cells of fundic glands in“experimental spleen deficiency group”were less than those of the normal control group,and LDH reactivity was elevated as compared with the normal control group.These enzyme activitied of spontaneous recovery group appeared similar to them of the“experimental spleen deficien- cy”group.The enzyme activities of the therapeutic group seemed to be similar to the normal control group.The activities of Mg^(2+)-ATPase and ALP in all the four group did not show any difference.Our study suggested that these changes of enzyme activities may be involved in pathogeneses of digestive disorders presented in spleen deficiency syndrome and the Chi- nese herbs(Si Jun Zi decoction)possessed significant therapeutic effect.

关 键 词：脾虚证 酶组织化学 胃粘膜 实验性 

分 类 号：R256.302[医药卫生—中医内科学]