RAPD法对50株幽门螺杆菌的分子流行病学  

A molecular epidemiological study of 50 Helicobacter pylori using RAPD

作　　者：代敏[1] 段广才[1] 郗园林[1] 范清堂[2] 李倩[1] 施侣元[3] 

机构地区：[1]河南医科大学公共卫生学院流行病学教研室,河南郑州450052 [2]河南省分子医学重点学科开放实验室,河南郑州450052 [3]同济医科大学公共卫生学院流行病学教研室,湖北武汉430030

出　　处：《疾病控制杂志》2002年第1期12-14,共3页Chinese Journal of Disease Control and Prevention

基　　金：河南省医学科技创新人才基金资助 (2 0 0 0 - 84 ) ;河南省分子医学重点学科开放实验室资助

摘　　要：目的　从基因水平研究幽门螺杆菌与疾病的关系。方法　用随机扩增的多态性 DNA(RAPD)法对 5 0株来自不同病人的幽门螺杆菌进行基因分型 ,并在此基础上探讨不同基因型别的幽门螺杆菌与疾病之间的关系。结果　 5 0株幽门螺杆菌均呈现不同的基因图谱 ,经聚类分析可分为 3个基因型别 ,此 3个基因型别在不同疾病中的发生率不同 ,经统计学分析 ,差异有显著性 (P=0 .0 3)。Objective To explore the relationship between the genetic types of Helicobacter pylori and different gastrointestinal diseases. Methods A random amplified polymorphic DNA(RAPD) fingerprinting method was used. Results 50 isolates of Hp showed 50 different fingerprints. Through the cluster analysis,they could be classified into three genetic types. Type Ⅰ was dominant in non ulcer group. Type Ⅲ was dominant in ulcer group. There was obvious difference between two groups (P=0.03). Conclusions Genetic types of Helicobacter pylori had the relationship with different gastrointestinal diseases.

关 键 词：随机扩增多态DNA技术 幽门螺杆菌 流行病学 分子 

分 类 号：R378[医药卫生—病原生物学]