山莨菪碱对大鼠热应激的影响  

Influence of anisodamine on heat-stress in rats

作　　者：唐文春[1] 王葆芳[1] 徐晓波[1] 刘广超[2] 段永建 

机构地区：[1]河南大学医学院物理教研室,开封475001 [2]河南大学医学院生化教研室,开封475001 [3]河南大学医学院一附院X-刀治疗中心,开封475001

出　　处：《中华劳动卫生职业病杂志》2002年第1期16-18,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的　探讨预先静脉注射山莨菪碱有无预防大鼠中暑性内毒素血症的作用。方法　实验动物随机分为两组 ,置于 38℃～ 40℃、5 0 %～ 6 0 %相对湿度的热环境中 ,用四道生理记录仪、半导体温度计测平均动脉压和直肠温度 ,并测热应激的生存时间和生存率 ,在严格无菌和去热源的条件下 ,采集门静脉血和颈动脉血 ,用显示基质偶氮法测血浆内毒素含量。结果　热应激过程中 ,实验组和对照组动物肛温持续增加 ,2h后分别达 (4 2 .7± 0 .6 )℃和 (4 3.1± 0 .5 )℃ ,两组比较 ,差异无显著性(P >0 .0 5 ) ,死亡前肛温高达 (4 4.6± 0 .2 )℃和 (4 4.2± 0 .3)℃ ,差异有显著性 (P <0 .0 1)。实验前门静脉血内毒素含量分别为 (4 5 .7± 5 .2 ) pg/ml和 (4 2 .6± 5 .4) pg/ml,外周血内毒素含量分别为 (14.8± 4.5 ) pg/ml和 (13.9± 7.2 )pg/ml,差异均无显著性 (P >0 .0 5 ) ;2h后门静脉血分别增至 (12 2 .2±16 .7)pg/ml和 (15 0 .4± 2 2 .1) pg/ml,而外周血增至 (32 .3± 7.8) pg/ml和 (4 9.7± 10 .2 )pg/ml,明显高于实验前 ,差异有显著性 (P <0 .0 1) ,而且两组之间比较 ,差异也有显著性 (P <0 .0 1)。两组动物心率变化趋势基本相同 ,都是先升后降 ,但实验前后心率变化的差异无显著性 (P >0 .0 5 ) ;实验前两组动?Objectives To study whether previously intravenous injection of anisodamine can prevent endotoxemia of heat stroke of rats. Methods Experimental animals were randomly divided into two groups,their average artery pressure,heart rate,survival time,survival rate and rectal temperature were measured at an environmental temperature of 38 ℃～40 ℃ and 50%～60% retative humidity.Blood samples for endotoxins analyses were taken both before and after heat-stress. Results During heat stress,the animals of rectal temperature of the experimental and control groups continuously increased and two hours later,separately to (42.7±0.6)℃ and (43.1±0.5)℃,without statistic difference(P>0.05),and to (44.6±0.4)℃ and (44.2±0.3)℃ prior to death,with statistic difference(P<0.05).Before the experiment,the contents of endotoxins of portal vein blood were (45.7±5.2)pg/ml and (42.6±5.4)pg/ml,and that of systemic blood was (14.8±4.5)pg/ml and (13.9±7.2)pg/ml,without statistic difference(P> 0.05).Two hours later,the contents of portal vein blood separately increased to (122.2±16.7)pg/ml and (49.7±10.2)pg/ml,obviously higher than that before heat-stress(P<0.01).And there were clear statistic difference between the two groups(P<0.01).The changing tendency of the heart rhythm is almost the same in two groups,that is,first rose and then fell.But it is without statistic difference before and two hours later(P>0.05):before heat-stress,the average artery pressures were (13.3±0.6)kPa and (13.6±0.5)kPa,without statistic difference(P>0.05),and two hours later,were (9.6±0.5)kPa and (8.6±0.6)kPa,with obvious statistic difference(P<0.01).The survival time of the animals were (166.5±16.9)min and (144.5±18.2)min with obvious statistic difference(P<0.01),the survival rate of heat stressed rats in the experimental group were obviously higher than control group(P<0.01 or P<0.05). Conclusions Anisodamine can prevent endotoxemia in rats suffering heat stroke.

关 键 词：中暑 热应激 山莨菪碱 内毒素血症 大鼠 

分 类 号：R965[医药卫生—药理学]