呼吸道合胞病毒下呼吸道感染T_H亚群功能状态的研究  

Study of T-helper cell function in infants with respiratory syncytial virus lower respiratory tract infection

作　　者：张艳敏[1] 雷春莲[1] 杨玉琮 阎辉[1] 成革胜[1] 

机构地区：[1]西安交通大学第一医院儿科,陕西西安710061

出　　处：《免疫学杂志》2002年第2期139-141,共3页Immunological Journal

基　　金：陕西省卫生厅基金资助项目(97062)

摘　　要：目的探讨RSV下呼吸道感染TH亚群功能状态。方 法用单克隆抗体双色免疫荧光PE/FITC双标记，流式细胞仪检测30例RSV下呼吸道 感染患儿急性期外周血单个核细胞（PBMCs）辅助性T淋巴细胞CD4+及其亚群CD4+CD 45RA+、CD4+CD45RO+的表达，ELISA方法检测血清中IFN-γ, IL-4, IgE 水平。结果RSV下呼吸 道感染组患儿外周血辅助性T淋巴细胞CD4+明显低于对照组(P<0.05)， CD4+CD45 RA+细胞明显下降(P<0.05)，CD4+CD45RO+细胞虽有下降，但与对照组相比 无统计学差异(P>0.05)。CD4+CD45RA+／CD4+CD45RO+比值与对 照组相比明显降低(P<0.05)。RSV下呼吸道感染组患儿血清中IFN-γ, IL-4水平均有 降低, 其中IFN-γ水平下降非常明显(P<0.01), IFN-γ/IL-4比值降 低(P<0.05)。RSV下呼吸道感染组患儿血清中IgE水平明显升高。结论婴幼儿RSV下呼吸道感染急性期的免疫功能紊乱主要表现为辅助性T淋巴细胞CD4+及其 亚群CD4+CD45RA+／CD4+CD45RO+的失衡。TH1及其功能下降，T H2及其功能相对增强，TH1/TH2失衡是导致该病发生TH2样反应重要的免疫机制。ObjectiveTo study the state of T-helper cell CD 4+ subpopulation in PBMCs infants with RSV lower respiratory tract infections. Methods 30 patients with RSV LRTIs CD4CD45RA, CD4CD45RO in PBMCs were analyzed by flow cytometry analysis. IFN-γ, IL-4, IgE levels were measured in serum by ELISA and compared with those in control group. Results In RSV(+) infants CD4+ cells decreased greatly(P <0.05), and CD4+CD45RA+, CD4+CD45RO+ all decreased, but level of CD4+CD45RO+ was not obvious as compared with control(P>0.05). TH1/TH2 ratio decreased in RSV(+) infants(P<0.05). For IFN-γ, IL-4 levels in serum, in RSV(+) group IFN-γ, IL-4 both decreased markedly(P<0.05). IFN-γ level decreased greater than IL-4 level. IFN-γ/IL-4 ra tio decreased in RSV(+) group. IgE level increased in RSV(+) group. Co nclusionsIn PBMCs of RSV infants, there is a disorder of immunology. T H1/TH2 unbalance occurs. TH2 takes high function relatively. T he immunoreaction type is TH2 type in RSV severe infection.

关 键 词：呼吸道合胞病毒 辅助性T淋巴细胞 TH1/TH2失衡 下呼吸道感染 婴幼儿 免疫学机制 

分 类 号：R725.6[医药卫生—儿科]