4-细胞胚胎卵裂球全能性的体外实验研究  

Investigation on development potential of blastomeres isolated from 4-cell human embryos

作　　者：高士友[1] 孙伟[1] 闻姬[1] 管群[1] 张琪瑶[1] 

机构地区：[1]济南铁路中心医院生殖中心,250001

出　　处：《中华妇产科杂志》2002年第3期155-156,W002,共3页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的　对体外培养的 4 细胞人类胚胎卵裂球发育及分化情况进行观察和研究。方法　采用控制性促超排卵获取人类卵子 ,体外受精获得早期胚胎 ,待发育到 4 细胞阶段时将透明带去除 ,分散成为单个卵裂球进行培养 ,观察卵裂球发育及分化的情况。结果　 2 0个 4 细胞、2个 3 细胞人类早期胚胎分散后获得单个卵裂球 83个 ,经体外培养后有 35个发育成为扩张囊胚。结论　 4Objective To investigate the development potential of blastomeres isolated from 4 cell human embryos Methods Human oocytes were collected after controlled ovarian hyperstimulation and fertilized in vitro The zona pellucida of 4 cell stage embryos were removed and the embryos were separated to single blastomeres and cultured in vitro The development and differentiation of the blastomeres were observed Results 83 blastomeres were isolated from 20 4 cell and 2 3 cell human embryos Of those, 35 developed to expanded blastocyst Conclusion Blastomeres isolated from the 4 cell human embryos have the competence to develop to expanded blastocyst

关 键 词：囊胚泡 体外研究 4-细胞胚胎卵裂球 人类胚胎增殖 胚胎移植术 

分 类 号：R711.6[医药卫生—妇产科学]