硫酸二乙酯和紫外线对金霉素链霉菌的诱变育种  

The effect of diethyl sulfate and ultra violet light as mutagenic agents on culture selection of Streptomyces aureofaciens

作　　者：黄晶 刘峰 刘勇 

机构地区：[1]福州抗生素集团公司,福州350002

出　　处：《中国抗生素杂志》2002年第3期138-140,共3页Chinese Journal of Antibiotics

摘　　要：考察了硫酸二乙酯 (DES)和紫外线 (UV)对金霉素链霉菌进行诱变的效果。其中 2 %DES诱变剂对金霉素链霉菌无明显的致死效应 ,但在含有金霉素 2 2 0 μg/ml的培养基上筛选结果表明 ,DES对金霉素产生菌产生了较强的诱变效果 ,菌株正突变率达 5 2 %。对金霉素链霉菌进行紫外诱变的结果表明 ,在营养贫瘠培养基上筛选的诱变效果较营养丰富培养基上的效果好 ,且遗传特性稳定。Two percent of diethyl sulfate (DES) could not produce significant lethal effect on S.aureofaciens , but in culture medium containing chlortetracycline (220μg/ml), an apparent mutation effect was observed , and the positive mutation rate reached 52%. In the case of using ultra violet light as mutagenic agent in the selection of S.aureofaciens , the result was better in poor nutrient medium than in the rich one, and the cultrue hereditary characters were also more stable.

关 键 词：金霉素链霉菌 硫酸二乙酯 紫外线诱变 培养基 金霉素 

分 类 号：R392.2[医药卫生—免疫学] R978.14[医药卫生—基础医学]