己烯雌酚对小鼠子宫内膜层粘连蛋白表达的影响  

Effects of Diethylstilbestrol on the Expression of Laminin in Mouse Endometrium

作　　者：魏纪东[1] 张顺利[1] 靳萍[1] 廖媛[1] 赵瑾[1] 杨林松[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453002

出　　处：《河南师范大学学报（自然科学版）》2002年第1期112-114,共3页Journal of Henan Normal University(Natural Science Edition)

基　　金：中法先进技术合作项目 (B96 - 11)

摘　　要：研究己烯雌酚 (DES)影响下小鼠子宫内膜层粘连蛋白 (LN)的表达变化 .2 6只雌性昆明小鼠随机分为三组 :未孕对照组 ,小剂量 (0 .2 8μgDES /g体重 )注射组 ,大剂量 (1.1μgDES /g体重 )注射组 .用免疫组化SP法和图像分析技术进行LN定位、定量测定 .正常小鼠子宫内膜LN在上皮基膜、血管内皮基膜均有表达 .注射己烯雌酚后 ,LN表达部位基本不变 ,表达量有明显增加 (P <0 .0 5 ) ,并随己烯雌酚剂量增加而增强 (P <0 .0 5 ) .通过影响LN的表达 ,可能是雌激素调控胚泡着床的机理之一 .Objective: To investigate the effects of diethylstilbestrol on laminin in mouse endometrium during early pregnancy. Methods: Twenty-six female Kunming mice were divided into three groups at random: control group; low-dosage injected (0.28μg DES/g weight) group; big-dosage injected (1.10μg DES/g weight) group. LN in mouse endometrium was shown and measured with immunohistochemical SP method and image analysis system. Results: The expression of laminin was found in the basement membrane of epithelium, glands and blood vessels in uterine endometrium during early pregnancy. When injected with DES, the localization of laminin didn't change, while the quantity and strength of laminin increased with the dosage of DES (P<0.05).Conclusion: It might be one of the mechanisms how female hormone regulated blastocyst implantation by affecting the expression of LN.

关 键 词：己烯雌酚 层粘连蛋白 子宫内膜 免疫组织化学 图像分析 小鼠 胚泡着床 表达定位 

分 类 号：Q492.5[生物学—生理学]