单抗间接Dot-ELISA检测禽脑脊髓炎病毒抗原的研究  

Establishment of indirect Dot-ELISA with McAb for detection of AEV antigen

作　　者：秦卓明 杨建民[2] 赵继勋[2] 苏敬良[2] 

机构地区：[1]山东省家禽研究所,山东济南250100 [2]中国农业大学实验动物所,北京100094

出　　处：《畜牧与兽医》2002年第3期8-11,共4页Animal Husbandry & Veterinary Medicine

基　　金：山东省农科院青年基金资助课题 (1 998 2 0 0 1 )

摘　　要：利用制备的禽脑脊髓炎病毒 (AEV)单抗建立了检测AEV抗原的间接Dot ELISA方法 ,对AEV最低检出量为 1 6 0 8μg/mL。人工感染 1日龄SPF鸡 ,取发病鸡 (6～1 3日龄 )相应病料 ,各病料样本阳性检出率分别为 :脑 1 0 0 % ,胰 96 % ,十二指肠 98% ,腺胃 98%。对 40份临床自然发病鸡病料检测 ,阳性检出率为 92 %。对 1 0份AEV病原分离阳性样品 ,检测阳性率为 1 0 0 % 。The procedure of indirect Dot ELISA has been successfully established for detection of AEV antigen,and the detection limit of the AEV was 16 08 μg/mL,The one day old SPF chickens were infected with AEV(V R and Shandong isolates)by the way of brains and subcutaneous in neck jection,and the pathological tissues and organs infected were collected when they are 6～13 day old.It was shown that the positive rates of AEV antigen in brain,pancreas,duodenum,gland stomach are 100%,96%,98%,98%respectively.These organs are the major targets infected by AEV.Forty samples collected from field infected chickens having clinical signs were detected,and the positive rate of AEV was 92%.

关 键 词：禽脑脊髓炎 间接Dot-ELISA 单克隆抗体 抗原检测 诊断 

分 类 号：S852.65[农业科学—基础兽医学] S855.3[农业科学—兽医学]