籼稻基因转化中的组织培养系统研究  

Study on Culture System in Gene Transformation of Indica Rice

作　　者：吴建国[1] 陈双燕[1] 石春海[1] 樊龙江[1] 

机构地区：[1]浙江大学农学系,杭州310029

出　　处：《中国农学通报》2002年第1期36-39,48,共5页Chinese Agricultural Science Bulletin

摘　　要：以MS培养基为基础 ,通过改变植物激素配比和添加附加物 ,确定了适合籼稻愈伤组织的诱导、继代和再生的优化条件。用不同的抗生素与除草剂 (Bar)处理愈伤组织 ,揭示了愈伤组织与抗生素和除草剂之间的关系 ,确定筛选具有卡那霉素 (Kan)或Bar抗性标记的转化子筛选浓度。同时通过不同抗生素的组合 ,选择出抑制共培养后的籼稻愈伤组织表面残余农杆菌合适条件。Suitable media were optimized for Indica calli induction, subculture, selection and differentiation by regulating the ratio between plant hormones and adding some supplements After calli were treated by different antibiotic and Bar, the effects between antibiotic or Bar and Indica rice calli were obtained for screening rice transformation with Kanamycin or Bar resistance Optical conditions with different antibiotic were selected to restrain Agrobacterium tumefaciens lines which left on the surface of calli after co-incubated

关 键 词：籼稻 组织培养系统 基因转化 培养基 培养条件 

分 类 号：S511.210.3[农业科学—作物学] S336