NK-κB在EAN中的作用及鼻粘膜耐受的影响  

Role of NF-kB in EAN and the effetct of nasal tolerance

作　　者：李静[1] 肖波[1] 周文斌[1] 谢光洁[1] 

机构地区：[1]湖南长沙中南大学湘雅医院神经内科,长沙410008

出　　处：《中国免疫学杂志》2002年第3期179-183,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目 ( 39470 2 6 2 ;39870 90 9)

摘　　要：目的 :探讨核转录因子NF κB在EAN中的作用以及鼻粘膜耐受的影响。方法 :采用RT PCR和Western blot法检测Wistar大鼠脾脏淋巴细胞IFN γ、TGF β1、IL 10mRNA表达和P6 5蛋白表达。 结果 :IFN γmRNA在发病早期表达明显增加 ,恢复期下降 ;IL 10、TGF β1mRNA在恢复期表达最明显 (P <0 0 5 )。鼻粘膜耐受后大鼠发病率和发病程度减轻 ,IL 10、TGF β1mRNA表达增加 (P <0 0 5 )。P6 5蛋白表达与IL 10、TGF β1mRNA表达呈负相关 ,与IFN γmRNA表达呈正相关。 结论 :P6 5蛋白可能参与EAN的发病 ,鼻服抗原诱导T细胞产生IL 10、TGF β1,通过抑制P6 5蛋白表达这一途径 ,有效预防EAN发生。Objective:To explore the role of nuclear transcription factor and the effect of nasal tolerance on EAN.Methods:75 male Wistar rats were divided into five groups: Group NS, EAN, EANL, EANM, EANH. The expression of IFN-γ, TGF-β1, IL-10 mRNA and P65 protein were detected in spleen by using of RT-PCR and western blot.Results:The expression of IFN-γ mRNA were the highest at early phase, those of TGF-β1 and IL-10 augmented significantly at recovery phase. In Group EANM and EANH, the severity of clinical signs and the infiltration of inflammatory cells decreased. IFN-γ mRNA and P65 protein expression was lower than that in Group EAN, however, IL-10 and TGF-β1 mRNA increased markedly. At the same time, the expression of P65 protein were positively correlated with those of IFN-γ mRNA, negatively correlated with those of TGF-β1 and IL-10 mRNA.Conclusion:NF-κB may play a vital role in EAN. Nasal administration of antigen may effectively prevent Wistar rat from inducing EAN in some degree, which may associate with the inhibition of NF-κB.

关 键 词：实验变态反应性神经炎 吉兰-巴雷综合症 鼻粘膜耐受 IFN-Γ TGF-Β1 IL-10 NF-ΚB EAN 

分 类 号：R339.12[医药卫生—人体生理学]