壳聚糖作为组织工程软骨支架的实验研究  

Experimental study on chitosan as a scaffolds for tissue engineering cartilage

作　　者：夏万尧[1] 曹谊林[1] 商庆新[1] 刘彦春[1] 钟伟[2] 

机构地区：[1]200011,上海第二医科大学组织工程研究中心,上海市组织工程研究重点实验室,上海第二医科大学附属第九人民医院整形外科 [2]复旦大学高分子科学系

出　　处：《中华显微外科杂志》2002年第1期34-37,共4页Chinese Journal of Microsurgery

基　　金：国家自然科学基金资助 (3990 0 1 56)

摘　　要：目的　探索壳聚糖作为组织工程技术中软骨细胞培养支架的可行性。　方法　消化分离猪耳郭软骨细胞 ,接种于自制壳聚糖、壳聚糖 胶原复合多孔支架上培养 ,从光镜和扫描电镜观察其亲水性和对细胞吸附力 ,MTT检测软骨细胞在壳聚糖、壳聚糖 胶原上的黏附率及壳聚糖、壳聚糖 胶原复合多孔支架对细胞的增殖、功能的影响。　结果　软骨细胞能够在壳聚糖、壳聚糖 胶原支架上黏附、伸展、增殖和发挥正常功能 ,MTT测得细胞黏附率分别为 81 2 5 %和 87 5 0 % ,MTT测得软骨细胞在壳聚糖 胶原支架中的增殖能力较强。　结论　壳聚糖、壳聚糖 胶原复合多孔支架与软骨细胞具有较好的相容性 ,壳聚糖 胶原复合多孔支架更适合作为软骨组织工程中的细胞培养支架。Objective This experiment aimed to confirm whether man made porous chitosan scaffold is a appropriate scaffold for chondrocyte culutre of tissue engineering Methods Chondrocytes were seeded onto porous chitosan and chitosan collagen complex scaffolds for culture in a three dimensional environment The scaffolds in hydrophilia and adhesion to chondrocytes were observed with light microscope and scanning electron microscope The number of the cells attached to the scaffolds and the function of the cells were detected with MTT automated colormetric microassay Result Chondrocytes can multiple and secrete the matrix on the poros chitosan and chitosan collagensc scaffolds The cell adhesion rates were 81 25% and 87 50% respectively Conclusion Chitosan can be fabricated into a suitable three dimensional porous scaffold Porous chitosan collagen complex scafflold may be a more suitable scaffold for chondrocyte culutre of tissue engineering

关 键 词：组织工程 软骨细胞 壳聚糖 支架 细胞培养 

分 类 号：R680.2[医药卫生—骨科学]