柯萨奇病毒B4’致低硒鼠心肌损伤的实验研究  

The experimental research on myocardial injury induced by CVB_4' in Se-deficient mice

作　　者：曹丹阳[1] 周令望[1] 曾宪惠[1] 钟学宽[1] 张淑芹[2] 白 虓 

机构地区：[1]哈尔滨医科大学克山病研究所,黑龙江哈尔滨150086 [2]哈尔滨医科大学实验动物学部,黑龙江哈尔滨150086 [3]哈尔滨市南岗区卫生防疫站,黑龙江哈尔滨150001

出　　处：《中国地方病学杂志》2002年第2期100-102,共3页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金资助项目(39670650);卫生部科学研究基金项目(96-1-3)

摘　　要：目的 探讨柯萨奇病毒B4’致低硒鼠心肌损伤的特点。方法 用补硒和低硒饲料分别喂养昆明鼠4周后交配,给其子代雄性鼠（出生后 4周）腹腔接种柯萨奇病毒B4’0.1ml,对照组接种等量RPMI 1640培养液。观察各组鼠的心肌光镜结果,全血中谷胱甘肽过氧化物酶（GSH-Px）活性和肝脏组织中脂质过氧化物酶（LPO）含量。结果 低硒和补硒饲料病毒组的心肌病变检出率分别为78.1％和16.7％。低硒和补硒饲料对照组的心肌未见病变。低硒组全血中GSH-Px活性明显降低,LPO含量明显增高。结论 低硒可以使柯萨奇病毒B4’致昆明鼠心肌损伤加重。Objective To investigate the characteristics of Se-deficient mice's myocardial injury induced by CVB4'. Methods The original Kunming mice were fed with Se-supplemented feed and Se-deficient feed respectively and mated after four weeks. The four-week male mice were inoculated intraperitoneally with CVB4',while control group was inoculated with equal-quantity of RPMI 1640 in the same way. Myocardial changes of mice in four groups were observed through light microscope, and the activity of GSH-Px in whole blood and the content of LPO in liver were also observed. Results The detection rate of myocardial lesion in Se-deficient virus group was 78. 1%,and that of Se-supplemented virus group was 16. 7%. The myocardium of the Se-deficient and Se-supplemented groups had no pathological changes. Activities of GSH-Px in whole blood of Se-deficient group were obviously reduced, and the content of LPO in liver was increased. Conclusions Se-deficiency can exacerbate or aggravate myocardial injury induced by CVB4' in Kunming mice.

关 键 词：柯萨奇病毒B4' 低硒 心肌损伤 谷胱甘肽过氧化物酶 克山病 

分 类 号：R542.3[医药卫生—心血管疾病] R373[医药卫生—内科学]