多色荧光原位杂交技术在精子染色体研究中的应用  

Chromosomal Analysis of Human Sperm Using Multiple Fluorescence in situ Hybridization

作　　者：张群芳[1] 卢光琇[1] 

机构地区：[1]中南大学湘雅医学院人类生殖工程研究室，湖南长沙410078

出　　处：《中华男科学杂志》2002年第1期54-56,共3页National Journal of Andrology

基　　金：国家重点基础研究发展规划项目 973课题 (G19990 5 5 90 1)

摘　　要：随着胞质内精子显微注射 (ICSI)的应用 ,严重少、弱、畸精子症病人也能获得治疗。现有许多研究发现这部分病人精子染色体非整倍率增加。如非整倍体的精子受精 ,产生的子代将导致流产、畸胎、死胎 ,因此对精子染色体数目的研究已成为男性不育精子检测的一项重要内容。近年来 ,多色荧光原位杂交 (FISH)技术在检测精子染色体非整倍体方面取得了一些进展 。Now severe oligoasthenoteratozoospermic(OAT) patients could have offsprings because of the development of technique of intracytoplasmic sperm injection. But some researchers found these patients have increasing frequency of the aneuploid on the chromosome in their sperm. If the spermatozoa with chromosomal aneuploid were fertilized,it would be resulted in a higher rate of recurrent abortion, fetal abnormal and dead birth,so the analysis of the number of sperm chromosome will play an important role in detection on infertile men. There are many new development in the chromosomal analysis of human sperm using multi FISH,now we have a review on them.

关 键 词：精子 染色体 非整倍体 多色荧光原位杂交 男性不育 

分 类 号：R698.2[医药卫生—泌尿科学]