川芎嗪对局灶性脑缺血多形核白细胞功能的影响  

Effects of Ligustrazine on Polymorphonuclear Leukocyte in Focal Cerebral Ischemia Cases

作　　者：张兆辉[1] 余绍祖[1] 赵保路[2] 

机构地区：[1]武汉大学人民医院,武汉430060 [2]中国科学院生物物理研究所

出　　处：《浙江中西医结合杂志》2002年第3期154-156,共3页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：湖北省科委实验项目 :NO.981916 0 2

摘　　要：目的 :研究川芎嗪 (Lig)对大脑中动脉梗塞区多形核白细胞 (PMNLs)功能的影响。方法 :采用大鼠局灶性脑缺血再灌注线栓模型 ,电子自旋共振自旋捕集技术和 Nelson氏法 ,观察 Lig对梗塞范围 ,PMNLs聚集和附壁现象以及对 PMNLs趋化性和呼吸爆发产生的氧自由基的影响。结果 :在体内实验 ,Lig组的梗塞体积 ,血管内 PMNLs聚集和附壁现象较对照组明显减少 (P均 <0 .0 5 )。在体外实验 ,Lig能清除 PMA刺激 PMNLs呼吸爆发产生的氧自由基并使 PMNLs趋化性发生抑制 ,其抑制率为 76 %。结论 :Lig可能通过抑制局灶性脑缺血 PMNLs趋化、聚集、吸附功能和清除氧自由基 。Objective: To study the effects of Ligustrazine on polymorphonuclear leukocytes (PMNLs) in infarction area supplied by the middle cerebral artery. Methods:Rat model with focal cerebral ischemia and reperfusion was employed and Nelson method was used to observe the infarction scope, PMNLs gathering and adhesion on the vascular wall,chemotaxis of PMNLs and oxygen free radicals. Results: In Vivo, the infarction scope, PMNLs gathering and adhesion on the vascular wall were resuced in Ligustrazine group (P<0.05). In vitro, Ligustrazine could remove the oxygen free radicals caused by PMA stimulation to PMNLs and inhibit the chemotaxis (The inhibition rate was 76%). Conclusion:Ligustrazine could protect the focal ischemic area by inhibting chemotaxis of PMNLs and clearing the oxygen free radicals.

关 键 词：局灶性脑缺血 多形核白细胞 氧自由基 川芎嗪 

分 类 号：R285.5[医药卫生—中药学]