脐血HLA-AB分型方法探讨  

Studies on the method of HLA-A,B Typing on Cord Blood

作　　者：兰炯采[1] 孙倩[2] 陈强[3] 张志梅[1] 武大林[1] 夏荣[1] 王从容[1] 

机构地区：[1]第一军医大学南方医院,广东广州510515 [2]北京医科大学附属人民医院 [3]中国医学科学院输血研究所

出　　处：《中国输血杂志》2002年第1期8-11,共4页Chinese Journal of Blood Transfusion

基　　金：四川省脐血干细胞中心项目;四川省卫生厅 (川卫科 970 0 5四川省科委 1997 14 75)资助

摘　　要：目的　探讨脐血HLA AB分型方法。方法　分别采用血清学方法和PCR SSP基因定型方法对 1 0 2份脐血HLA A、B位点进行检测。结果　两方法对比分析结果显示血清学近 1 8.6 %不能得出满意结果 ,HLA A、B位点错定率分别为 6 .1 %和 1 0 .8% ,总错误率 1 6 .9%。结论　脐血HLA表达水平低 。Serological typing for HLA A,B has been used for a long time. Recently with the development of molecular biology technologies, HLA A,B typing is now turning to genotyping methods. In our study, we evaluated the capacity of PCR SSP in solving problems in HLA A,B typing with serological methods.With this aim we compared serology with PCR SSP in 102 cord blood samples, it showed that 18.6% of serdogical typing of 102 cord blood samples couldn't give a satisfactory result,Discrepant test results between the 2 methodsoccured in 14 samples. It is mainly due to the weak expression of HLA class I cord blood Iymphocytes and the cross reaction of some antigens. Our study shows clearly that DNA typing is better than serological typing for cord blood.

关 键 词：脐血 分型方法 HLA-A、B PCR-SSP法 血清学方法 脐血移植 

分 类 号：R457.1[医药卫生—治疗学]