高效液相色谱法测定仙茅中仙茅苷的含量  

Determination of Curculigoside in Crude Medicine Curculigo orchioides by HPLC

作　　者：陆惠文[1] 朱炳辉[1] 梁颖康[1] 

机构地区：[1]广东省药品检验所,广东广州510180

出　　处：《中国中药杂志》2002年第3期192-194,共3页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金资助项目 ( 2 96 32 0 5 0 )

摘　　要：目的 :建立中药材仙茅中仙茅苷含量的反相高效液相色谱法。方法 :甲醇为溶剂 ,超声提取 ,Sep PakC18柱净化 ,色谱柱IntertsillODS 3,流动相甲醇 水 冰醋酸 (4 5∶80∶1) ,流速 1.0mL·min-1,检测波长 2 83nm。结果 :平均回收率为 99.2 % ,RSD =1.7% (n =5 ) ,6个不同来源的仙茅药材中仙茅苷的含量在 0 .11%～ 0 .35 %。结论 :可作为仙茅药材的质量控制方法。Objective:To establish a method for the determination of curculigoside in Curculigo orchioides ,a species of crude medicine.Method:The methanol was used as the solvent of exatraction and the curculigoside was extracted from the crude medicine with the method of the ultrasonic vibration. Using Sep-Pak C 18 cartridges to purify the solution,the curculigoside was detected by HPLC. Intersil ODS-3(150 mm×4.6 mm,5 μm) chromatographic column was used, mobile phase of methanol-water-ice acetic acid(45∶80∶1)and detect wavelength was set at UV 283 nm.Result:The average recovery was 99.2% for the detemination of curculigoside,RSD=1.7%( n =5). The content range of the curculigoside for 6 kinds of different samples was from 0.11% to 0.35%.Conclusion:This method could be used to control the quality of crude medicine C. orchioides .

关 键 词：仙茅 仙茅苷 高效液相色谱法 固相萃取 含量测定 

分 类 号：R284.1[医药卫生—中药学] R282.71[医药卫生—中医学]