腹膜透析液对腹腔巨噬细胞产生一氧化氮和细胞活力的影响  

Influence of peritoneal dialysate on nitric oxide production and viability of macrophage

作　　者：杨泽然[1] 李继承[1] 张凯[2] 宓旭峰[1] 

机构地区：[1]浙江大学医学院,浙江杭州310031 [2]杭州市红十字会医院,浙江杭州310004

出　　处：《浙江大学学报（医学版）》2002年第1期23-25,共3页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省科委分析测试基金项目

摘　　要：目的 :探讨商业性腹膜透析液 (CDS)对巨噬细胞功能的影响。方法 :培养的小鼠腹腔巨噬细胞 (Mφ)分为两大实验组及各自的对照组 :1将 Mφ置于含 5 0 % CDS(含糖 0 .139mol/ L)的条件培养液中培养 2 4 h;2 Mφ分别暴露于含 0 .139mol/ L糖浓度 CDS中 10、30和 6 0 min,然后在无 CDS培养液中培养 2 4 h。观察 Mφ的一氧化氮(NO)产量和 MTT还原能力。对照组以等量培养液代替 CDS。结果 :实验组与对照组比较 ,Mφ的 NO产量和 MTT还原能力均受明显抑制 ;CDS暴露 6 0 min组比暴露 10、30 min组所受抑制作用强。结论 :腹膜透析液可损害巨噬细胞活力和降低其 NO产量 。Objective: To investigate the influence of commercial peritoneal dialysate(CDS) on function of macrophage.Methods: Cultured peritoneal macrophages were divided into two experimental groups and their controls. ①Macrophages were cultured in conditioned culture medium containing 50% CDS (0.139 mol/L glucose) for 24 h. ②Macrophage were exposed to CDS containing 0.139 mol/L glucose for 10, 30 and 60 min respectively, and then cultured in CDS free medium for 24 h. The nitric oxide (NO) production and MTT in two groups were measured. In each control group, CDS was replaced by same amount of culture medium. Results: NO production and MTT reduction ability (related to cell viability) of experimental groups were remarkably lower than those of controls and the NO production and MTT reduction in 60min CDS exposed group were lower than that of 10 min and 30 min CDS exposed group. Conclusion: Dialysate may have detrimental effects on viability and other function of macrophage and these effects may related to time length of CDS exposure.

关 键 词：腹膜透析 透析液 腹腔巨噬细胞 一氧化氮 小鼠 CDS 细胞活力 

分 类 号：R459.5[医药卫生—治疗学] Q813[医药卫生—临床医学]