党参、黄芪、白术、甘草提取部位对小肠上皮细胞增殖的影响  

Effects of extracts from Codonopsis pilosula, Astragalus membranaceus, Atractylodes macrocephala and Glycyrrhiza uralensis on intestinal crypt cells proliferation

作　　者：张子理[1] 陈蔚文 

机构地区：[1]广州中医药大学脾胃研究所,广州510405

出　　处：《中药药理与临床》2002年第1期10-12,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金 (No .39970 90 6);广东省自然科学基金 (No .990 354);广东省千百十人才工程基金 (No.9953)项目

摘　　要：目的 :观察党参、黄芪、白术和甘草提取部位对IEC 6细胞增殖的作用。方法 :IEC 6细胞培养 2 4h后分别加入不同剂量的党参、黄芪、白术和甘草的各种提取部位 10 μl,2 4h后用MTT法观察细胞增殖。 结果 :不同提取部位对小肠上皮细胞增殖的作用各异 ,有些部位促进细胞增殖 ,有些部位抑制细胞增殖 ,有些部位无明显影响。同一部位剂量不同其作用方向也不同。结论 :益气健脾药物通过调节小肠上皮细胞增殖发挥其粘膜保护和修复作用。Aim: To characterize the role of the extracts from Codonopsis pilosula (CP), Astragalus membranaceus (AM), Atractylodes macrocephala (AMC) and Glycyrrhiza uralensis (GU) on the proliferation of intestinal crypt cells (IEC 6) in vitro. Methods: IEC 6 were plated in 96 well microplates for 24h and then were treated with 10μl the extracts from CP, AM, AMC and GU. The proliferative capability of the cells was estimated at 24h after treatment in extracts groups by MTT assay at aborbance 570nm. Results: These results indicated that various extracts from CP, AM, AMC and GU, and their various dosage had different effects on IEC 6 cell proliferation. Some promoted proliferation, some inhibited proliferation, and some had no effect, and same extract also had different effects at different dosage. Conclusion: It is suggested that CP, AM, AMC and GU promoted mucosal repair by regulating IEC 6 cell proliferation.

关 键 词：IEC-6细胞株 党参 黄芪 白术 甘草 细胞增殖 胃肠粘膜损伤 

分 类 号：R285.5[医药卫生—中药学]