Direct detection of oxygen free radicals produced in the viscera of burned rats using electron paramagnetic resonance spectroscopy  

作　　者：路卫 陈玉琳 夏照帆 

出　　处：《Chinese Journal of Traumatology》2002年第2期118-120,共3页中华创伤杂志（英文版）

基　　金：SupportedbytheNationalNaturalScienceFoundationofChina (No .3 880 791)

摘　　要：To detect superoxide anion (O - 2 ) signals in the heart, liver, lung and kidney tissues after burns. Methods: Twenty four male rats were randomized into 4 groups. The rats of experimental groups were immersed in 100℃ water for 15 seconds and 25% third degree burn was created. Thoracotomy or laparotomy was performed at 5, 10, 15, 20 and 30 minutes after burn, and specimens of the heart, lung, liver kidney were obtained for burned rats. The specimens were then preserved in liqu id nitrogen for cryo preservation and detected immediately using EPR. Results: The signals of superoxide O radical appeared in the he art, lung, 1iver and kidney specimens 10 15 minutes after burn. Conclusions: There is a direct evidence of oxygen free radicals (OFRs) injury to viscera of burned rats between 10 15 minutes after burn.

关 键 词：BURNS Oxygen free radicals Electron paramagnetic r esonance 

分 类 号：R644[医药卫生—外科学]