内毒素血症大鼠淋巴细胞中降钙素基因相关肽(CGRP)释放及mRNA表达的变化  

Changes of CGRP Levels in Plasma and CGRP mRNA in Lymphocytes during Endotoxemia in Rats

作　　者：宋继荣[1] 辛云霞[2] 邢宇彤[1] 

机构地区：[1]黑龙江省佳木斯大学第一附属医院,154002 [2]桦川县人民医院

出　　处：《中国误诊学杂志》2002年第3期346-349,共4页Chinese Journal of Misdiagnostics

摘　　要：目的　测定大鼠内毒素血症期间淋巴细胞释放和合成降钙素基因相关肽 (CGRP)的变化。方法　采用逆转录聚合酶链反应 (RT- PCR)测定大鼠淋巴细胞 CGRP m RNA水平。用放射性标记法测定血浆 CGRP水平。结果　注射内毒素大鼠血浆和淋巴细胞释放 CGRP均增高 ,淋巴细胞中 CGRP m RNA表达也明显增高。结论　提示内毒素不仅刺激淋巴细胞释放 CGRP,而且能通过某些机制激活淋巴细胞 CGRP m RNA的转录 ,使 CGRP合成增加 ,以作为 CGRP大量释放的重要补充来源。Objective To investigate the changes of both CGRP release and synthesis during endotoxemia.Method Expression of CGRP mRNA in lymphocytes was determined by the semi quantity RT PCR and the CGRP levels in plasma were measured by RIA.Results Signficantly increased CGRP release in plasma and CGRP expression in lymphocyte were foundr 3hr and 8hr after endotoxin(5mg/kg,i.v.) was administrated.Conclusion It suggested that endotoxin could not only stimulate the release of CGRP from lymphocytes,but also the transcription of CGRP mRNA and synthesis of CGRP in lymphocytes during the development of endotoxemia in rats,so lymphocytes might be an important source of CGRP release during endotoxemia.

关 键 词：内毒素血症 淋巴细胞 降钙素基因相关肽 mRNA 大鼠 RT-PCR 

分 类 号：R631.1[医药卫生—外科学]