中国不同人群的抗TTV血清流行病学及TTV基因不同区段的分子流行病学研究  

Sero-and molecular epidemilogy of the gene of different ORF of transfusion transmitted virus (TTV) in different population of China

作　　者：李伯安[1] 程云[1] 侯俊[2] 高蓉 李靖 郑宇[1] 苏琴[1] 付体权[1] 

机构地区：[1]解放军第三○二医院传染病研究所免疫研究室,北京100039 [2]解放军第三○二医院传染病研究所病毒研究室,北京100039

出　　处：《中华实验和临床病毒学杂志》2002年第1期79-81,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　了解不同人群血清中抗 TTV抗体及ORF1 、ORF2 区段基因的分布状况 ,并分析其间的关系。方法　根据TTV的ORF1 、ORF2 区段的基因序列分别合成巢式PCR引物 ,扩增 2 46例血清标本中的TTV部分基因片段 ;采用TTVORF2 部分基因原核表达抗原 ,应用酶联免疫吸附试验(ELISA) ,检测相同血清标本中TTV抗体。结果　不同人群TTVORF1 、ORF2 基因及抗体检测的阳性率分别为 :有偿献血者 16 0 % (12 75 ) ,10 7% (8 75 )和 2 5 3% (19 75 ) ,甲型肝炎患者 10 0 % (3 30 ) ,16 7% (5 30 )和 16 7% (5 30 ) ;乙型肝炎患者 47 5 % (19 40 ) ,42 5 % (17 40 )和 2 2 5 % (9 40 ) ,丙型肝炎患者 42 9% (15 35 ) ,37 1% (13 35 )和 2 8 6 % (10 35 ) ;丁型肝炎患者 2 0 0 % (3 15 ) ,2 6 7% (4 15 )和13 3% (2 15 ) ;戊型肝炎患者 16 7% (2 12 )、16 7% (2 12 )、33 3% (4 12 ) ;庚型肝炎患者 2 3 8% (5 2 1) ,38 1% (8 2 1)和 2 3 8% (5 2 1) ;非甲～庚型肝炎患者 6 1 1% (11 18) ,5 0 0 % (9 18)和 44 4% (8 18)。统计分析TTVORF1 与ORF2 基因的检出率相关有统计学意义 (P =0 0 0 0 <0 0 1) ;不同人群间基因检出率相差有统计学意义 (P <0 0 1) ;Objective To investigate the distribution of anti-TTV antibody and the different ORF gene in different populations of China.Methods The antibody to TTV in sera collected from different population were detected by using ELISA and the different ORF genes were amplified with PCR.Results The positive rates of TTV ORF 1 DNA,ORF 2 DNA and the antibody in various populations were as follows:16.0%(12/75), 10.7%(8/75) and 25.3%(19/75) in paid blood donors;10.0(3/30), 16.7%(5/30) and 16.7%(5/30) in patients with hepatitis A,47.5%(19/40),42.5%(17/40) and 22.5%(9/40) in patients with hepatitis B; 42.9%(15/35),37.1%(13/35) and 28.6%(10/35)in patients with hepatitis C; 20.0%(3/15),26.7%(4/15) and 13.3%(2/15)in patients with hepatitis D; 16.7%(2/12),16.7%(2/12) and 33.3%(4/12)in patients with hepatitis E; 23.8%(5/21),38.1%(8/21) and 23.8%(5/21) in patients with hepatitis G; 61.1%(11/18),50.0%(9/18) and 44.4%(8/18) in patients with non A-G hepatitis,respectively.The positive rate of different ORF DNA had no significant difference.Significant differences were found in the positive rates of TTV DNA in various populations.There was no relationship between the TTV DNA and the antibody to TTV.Conclusion The antibody to TTV and TTV DNA were found in every population of China.There was no significant difference in the positive rates of TTV DNA between ORF 1 and ORF 2.The positive rate in patients with non A-G hepatitis was higher than those in the other populations.

关 键 词：输血传播病毒 病毒抗体 酶联免疫吸附测定 聚合酶链反应 血清流行病学 

分 类 号：R181.3[医药卫生—流行病学]