高效液相色谱法同时测定血清中的舒乐安定和安定  

Simultaneous Determination of Estazolam and Diazepam in Serum With HPLC

作　　者：张丽[1] 何林[1] 孙世明[1] 谭静[1] 

机构地区：[1]四川省人民医院药剂科,成都市610072

出　　处：《中国药房》2002年第2期97-98,共2页China Pharmacy

摘　　要：目的 :高效液相色谱法 (HPLC)同时测定舒乐安定和安定的血清浓度。方法 :采用HPLC法 ,流动相为甲醇 -水 -三乙胺 -冰醋酸 (55∶45∶0 5∶0 35) ,检测波长为254nm ;以苯妥英为内标 ,样品在碱性条件下用乙醚萃取。结果 :舒乐安定的浓度在0 52～38 62μg/ml、安定的浓度在0 50～40 16μg/ml范围内线性关系良好。结论 :本法操作简便 ,能快速。OBJECTIVE:To establish HPLC method for simultaneous determination of estazolam and diazepam in serum METHODS:The mobile phase consisted of methanol-water-triethylamine-acetic acid(55∶45∶0 5∶0 35)and the λmax was 254nm Phenytoin was adopted as the internal standard The drugs were extracted by diethyl ether under the alkaline condition RESULTS:The calibrating curves of estazolam and diazepam were linear in the ranges of 0 52～38 62μg/ml and 0 50～40 16μg/ml respectively CONCLUSION:The method was appropriate for determination of estazolam and diazepam qualitatively and quantitatively in intoxication accident

关 键 词：高效液相色谱法 舒乐安定 安定 测定 血清 

分 类 号：R971.3[医药卫生—药品]