伪狂犬病病毒gG-gD基因片段的扩增及克隆和序列测定  

Amplification, Cloning and Sequencing of a Fragments of G and D Gene of Pseudorabies Virus

作　　者：罗满林[1] 黄毓茂[1] 刘镇明[1] 吴红专[1] 袁少华[1] 

机构地区：[1]华南农业大学动物医学系,广东广州510642

出　　处：《中国兽医科技》2001年第8期3-5,共3页Chinese Journal of Veterinary Science and Technology

基　　金：国家自然科学基金资助项目 (39770 0 33) ;广东省科技攻关项目 (2 0 0 0 - 2 0 0 1 )的部分内容

摘　　要：以伪狂犬病病毒 (PRV)HB 93 0 4株的基因组为模板 ,利用聚合酶链反应 (PCR)对其gG gD基因片段进行扩增 ,获得了预定大小的片段 ,将这一片段克隆到质粒载体 pPK中。对重组质粒pPKGD进行限制性内切酶分析、PCR鉴定和克隆片段的序列测定 ,证实了克隆片段的可靠性。Using the genome DNA of the strain HB 9304 of Pseudorabies virus (PRV) as template, the fragments flanking gG and gD were amplified by polymerase chain reaction(PCR) .The expected size of fragments were obtained ,and then cloned into the plasmid pPK. The recombinant plasimd of pPKGD was identified by restriction enzyme analysis,PCR and sequencing, which proved completely its validity.

关 键 词：伪狂犬病病毒 聚合酶链反应 gG-gD基因片段 分子克隆 序列测定 

分 类 号：S852.659.1[农业科学—基础兽医学]