凤宝胶囊质量标准的研究  

Quality control for Fengbao Capsule by HPLC and TLC respectively

作　　者：陈叶青[1] 李国强[1] 刘永刚[2] 

机构地区：[1]广州市花城制药厂,广东广州510555 [2]第一军医大学药理教研室,广东广州510555

出　　处：《湖南中医药导报》2002年第2期50-51,共2页Hunan Guiding Journal of Traditional Chinese Medicine and Pharmacology

摘　　要：目的 :建立凤宝胶囊中的当归、丹参等成分薄层鉴别和芍药苷含量测定的方法。方法 :采用TLC和HPLC。在HPLC中色谱柱为C18柱 ,流动相为甲醇 -水 (2 5 :75 ) ,检测波长为 2 30nm。TLC用阴阳对照试验 ,对方中的两种成分进行确认。结果 :当归、丹参供试品色谱中在与对照药材色谱相应的位置上显相同颜色的斑点。HPLC测定芍药苷的含量 ,芍药苷在 0 .336～ 1.6 80 μg范围内呈线性关系 ,平均回收率为 98.8% ,RSD =1.6 %。结论 :该方法简便、稳定 。Objective: To establish a method for the idenfification of Chinese angelica, Dan-shen root, and the content lactiflorin in Fengbao Capsule. Method: Applying HPLC and TLC respectively. As for HPLC, C 1 8 was used for column, methanol-water (25:75) for mobile phase, detective wave 278 nm. Positive and negative contrasts was selected for making sure the Chinese angelica and Dan-shen root in TLC assay. Results: For samplesplution of Fengbao Capsule's, the same color appeared in the spot where the positive contrast material occurred. For the lactiflorin in Fengbao Capsule by way of HPLC, the calibration curve was linear within 0.336～1.680 μg. The average recovery rate of lactiflorin was 98.8%, with RSD=1.6%. Conclusion: The method is simple and stable, and can be used for the quality control for Fengbao Capsule.

关 键 词：凤宝胶囊 薄层色谱法 高效液相色谱法 当归 丹参 芍药苷 质量标准 

分 类 号：R286.0[医药卫生—中药学]