腺病毒介导的人p27kip1基因转染对球囊损伤后兔颈动脉新生内膜增生的抑制作用  

Adenovirue-mediated human p27kip1 gene transfection inhibits neointimal proliferation of rabbit carotid artery after balloon injury

作　　者：潘晓明[1] 吴宗贵[1] 章卫平[2] 金海[2] 曹雪涛[3] 

机构地区：[1]上海第二军医大学附属长征医院心内科,上海200003 [2]第二军医大学免疫学教研室 [3]第二军医大学附属长海医院心胸外科

出　　处：《中国介入心脏病学杂志》2002年第1期44-46,共3页Chinese Journal of Interventional Cardiology

基　　金：国家杰出青年科学基金(39825123)

摘　　要：目的 研究腺病毒介导的p27kip1基因及其蛋白产物高表达对球囊损伤后兔颈动脉新生内膜增生的抑制作用。方法 建立兔颈动脉球囊损伤模型,将LacZ重组腺病毒（AdLacZ）和人p27kipl重组腺病毒（Adhp27kipl）在体内分别转染损伤动脉节段,以Western　blot、x-gal染色、HE染色、兔疫组化及计算机图像处理方法观察转染动脉节段中外源性p27kipl蛋白表达及其对新生内膜增生的影响。结果 与AdLacZ转染组及未转染组比较,Adhp27kip1转染的动脉节段内p27kip1蛋白呈高表达,表达高峰在7～14 d,持续4周以上;转染4周后未转染组、AdLacZ转染组及Adhp27kip1转染组的新生内膜面积分别为1.106mm2、0.988mm2及0.278mm2;管腔狭窄率分别为87.07％、65.40％及32.14％。结论 外源性p27kip1基因及其蛋白产物在损伤动脉节段内高表达可显著抑制新生内膜增生及管腔狭窄。Objective To study the effects of adenovirue-mediated p27kipl gene and its protein product overexpression on neointimal proliferation of rabbit carotid artery after balloon injury. Methods After rabbit arterial carotid injuried, injuried arterial segments were immediately infected by LacZ recombinant adenovirues (AdLacZ) and human p27kipl recombinant adenovirues (Adhp27kip1) in vivo, respectively. Western blot, x-gal stainning, HE stainning, immunochemistry and compute image system were used to analyze the expression of exogenous p27kipl gene and its protein product and the effects on neointimal proliferation. Results Comparison to AdLacZ infected or uninfected arterial segments, Adhp27kip1 infected segments overexpressed p27kipl protein, the peak of expression was in 7-14 d, expression lasted more than 4 weeks. After 4 weeks, in uninfected, AdLacZ infected or Adhp27kipl infected segments, the neointimal area was 1.106 mm2, 0.988 mm2 and 0.278 mm2, respecively; the rate of luminal stenosis was 87.07%, 65.40% and 32.14%, respectively. Conclusion Exogenous p27kipl gene and its protein product overexpression in injuried arterial segments could inhibit neointimal proliferation and luminal stenosis significantly after artery injury.

关 键 词：P27KIPL 血管损伤 蛋白表达 细胞周期 腺病毒 基因治疗 

分 类 号：R456[医药卫生—治疗学]